Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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803 Results
  • Anti-MCT1 (SLC16A1) (extracellular)-mFluor™ Violet 450 Antibody
    Anti-MCT1 (SLC16A1) (extracellular)-mFluor™ Violet 450 Antibody
    A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 1 conjugated to mFluor™ Violet 450 fluorophore
    • Product Code: AMT-011-V
    • Short Description: A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 1 conjugated to mFluor™ Violet 450 fluorophore
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Monocarboxylate transporter 1, Monocarboxylic acid transporter 1
  • Anti-CRF2/CRHR2 (extracellular)-FITC Antibody
    Anti-CRF2/CRHR2 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Corticotropin-Releasing Factor Receptor 2 Conjugated to the Fluorescent Dye FITC
    • Product Code: ACR-052-F
    • Short Description: A Rabbit Polyclonal Antibody to Corticotropin-Releasing Factor Receptor 2 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Corticotropin-releasing factor receptor 2, CRF-R-2, CRF2 receptor beta isoform, Corticotropin-releasing hormone receptor 2, CRH receptor 2 variant B
  • Anti-Adenosine A<sub>2B</sub> Receptor (extracellular)-PE Antibody
    Anti-Adenosine A2B Receptor (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2B Receptor (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE) fluorophore.
    • Product Code: AAR-003-PE
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2B Receptor (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE) fluorophore.
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ADORA2B
  • Anti-Adenosine A<sub>2A</sub> Receptor (extracellular)-APC Antibody
    Anti-Adenosine A2A Receptor (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Allophycocyanin (APC) fluorophore
    • Product Code: AAR-008-APC
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Allophycocyanin (APC) fluorophore
    • Host: Rabbit
    • Reactivity: Rat, Mouse
    • Applications: Flow cytometry
    • Target: ADORA2A, A2AAR, Adenosine A2aR
  • Anti-GPR83 (extracellular)-FITC Antibody
    Anti-GPR83 (extracellular)-FITC Antibody
    A rabbit polyclonal antibody to GPR83 Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-053-F
    • Short Description: A rabbit polyclonal antibody to GPR83 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: G-protein coupled receptor 83, G-protein coupled receptor 72, GPR72, Glucocorticoid induced receptor, GIR
  • Anti-GPR41/FFAR3 (extracellular) Antibody
    Anti-GPR41/FFAR3 (extracellular) Antibody
    A rabbit polyclonal antibody to GPR41/FFAR3 (extracellular)
    • Product Code: AFR-017
    • Short Description: A rabbit polyclonal antibody to GPR41/FFAR3 (extracellular)
    • Host: Rabbit
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: FFAR3, GPR41, Free fatty acid receptor 3, G-protein coupled receptor 41
  • Anti-Na<sub>V</sub>1.7 (SCN9A) (extracellular) Antibody
    Anti-NaV1.7 (SCN9A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NaV1.7 (SCN9A) Channel
    • Product Code: ASC-027
    • Short Description: A Rabbit Polyclonal Antibody to NaV1.7 (SCN9A) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA
  • Anti-P2Y12 Receptor (extracellular)-PE Antibody
    Anti-P2Y12 Receptor (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to P2Y12 Conjugated to the Fluorescent Dye Phycoerythrin
    • Product Code: APR-020-PE
    • Short Description: A Rabbit Polyclonal Antibody to P2Y12 Conjugated to the Fluorescent Dye Phycoerythrin
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2Y purinoceptor 12, P2RY12, P2Y receptor
  • Anti-P2Y12 Receptor (extracellular)-FITC Antibody
    Anti-P2Y12 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to P2Y12 Receptor Conjugated to the Fuorescent dye FITC
    • Product Code: APR-020-F
    • Short Description: A Rabbit Polyclonal Antibody to P2Y12 Receptor Conjugated to the Fuorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3
  • Anti-P2X7 Receptor (extracellular)-FITC Antibody
    KO Validate'
    Anti-P2X7 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to P2X7 Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: APR-008-F
    • Short Description: A Rabbit Polyclonal Antibody to P2X7 Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Flow cytometry
    • Target: Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor
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