Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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796 Results
  • Anti-5HT1B Receptor (HTR1B) (extracellular) Antibody
    Anti-5HT1B Receptor (HTR1B) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1B
    • Product Code: ASR-022
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1B
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: 5-Hydroxytryptamine receptor 1B, Serotonin receptor 1B, Serotonin 1D beta receptor, 5HT1D-beta, HTR1DB, HTR1D2
  • Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody
    Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1B Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ASR-022-AG
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1B Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: 5-Hydroxytryptamine receptor 1B, Serotonin receptor 1B, Serotonin 1D beta receptor, 5HT1D-beta, HTR1DB, HTR1D2
  • Anti-5HT1D Receptor (HTR1D) (extracellular) Antibody
    Anti-5HT1D Receptor (HTR1D) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1D
    • Product Code: ASR-023
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1D
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: 5-Hydroxytryptamine receptor 1D, Serotonin receptor 1D, 5HT1D-alpha, HTR1DA
  • Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody
    Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2A
    • Product Code: ASR-033
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: 5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A
  • Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody
    Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2B
    • Product Code: ASR-035
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2B
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: 5-Hydroxytryptamine receptor 2B, Serotonin receptor 2B, 5-HT-2B, 5-HT2F, Stomach fundus serotonin receptor
  • Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody
    Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2B Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ASR-035-AG
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2B Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry, Immunohistochemistry
    • Target: 5-Hydroxytryptamine receptor 2B, Serotonin receptor 2B, 5-HT-2B, 5-HT2F, Stomach fundus serotonin receptor
  • Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody
    Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 3B
    • Product Code: ASR-032
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 3B
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: 5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B
  • Anti-5HT3B Receptor (HTR3B) (extracellular)-ATTO Fluor-488 Antibody
    Anti-5HT3B Receptor (HTR3B) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 3B Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ASR-032-AG
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 3B Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Rat, Mouse
    • Applications: Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: 5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B
  • Anti-5HT4 Receptor (HTR4) (extracellular) Antibody
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    Anti-5HT4 Receptor (HTR4) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4
    • Product Code: ASR-036
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry
    • Target: 5-Hydroxytryptamine receptor 4, Serotonin receptor 4
  • Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody
    Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4 Conjugated to the Fluorescent Dye FITC
    • Product Code: ASR-036-F
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: 5-Hydroxytryptamine receptor 4, Serotonin receptor 4
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