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Multiplex Staining Updates

Alomone Labs has developed two innovative platforms for studying protein-protein interaction and/or protein co-localization in complex immunostaining or immunoprecipitation protocols:

ATTO-Conjugated Antibodies
These antibodies can be used in immunohistochemistry (IHC) and immunocytochemistry (ICC) with same species antibodies.

Save time and resources with ONE antibody.

Benefits of immunostaining with ATTO-conjugated antibodies:
Direct immuno-fluorescence
Secondary antibodies are not necessary
Ideal for multiplex staining with same species/host antibodies

Guinea Pig-Raised Antibodies
Antibodies raised in guinea pig can be used with any non-guinea pig second species antibodies (e.g. antibodies raised in mouse, rabbit, goat, etc.) in complex immunostaining or immunoprecipitation protocols. Our line of guinea pig raised antibodies can be combined with any of our rabbit-raised or ATTO-conjugated antibodies in complex immunostaining protocols.

The complete list of Multiplex Staining Products.

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Multiplex Staining of TRPV4 and Aquaporin 2 in Mouse Kidney


Immunohistochemical staining of mouse kidney sections using Anti-TRPV4 Antibody (#ACC-034) and Anti-Aquaporin 2-ATTO Fluor-590 Antibody (#AQP-002-AO). TRPV4 staining (green) is detected in the tubules of the cortex and meddula (left panel). AQP2 immunoreactivity (red) is detected in the same areas (middle panel). Merged image shows complete overlap between TRPV4 and AQP2 staining. Adapted from Berrout, J. et al. (2012) J. Biol. Chem. 287, 8782. with permission of the American Society for Biochemistry and Molecular Biology.

Multiplex Staining of VGLUT2 and Neurokinin 1 Receptor in Rat DRG
Multiplex Staining of KCa3.1 and P2Y2 in Human Ovarian Carcinoma
Multiplex Staining of GluN1 and ASCT1 in Mouse Hippocampus
Multiplex Staining of GIRK2 and LRRK2 in Mouse Substantia Nigra
Multiplex Staining of GluN2A and SynGAP in Rat Cingulate Cortex
Multiplex Staining of Kir6.2 and BDNF in Mouse Hippocampus
Multiplex Staining of Aquaporin 4 and TrkB in Rat Cerebellum
Multiplex Staining of Synapsin-2 and GABA(A) α1 Receptor in Rat Striatum
Multiplex Staining of Slitrk1 and KV2.1 in Rat Parietal Cortex
Multiplex Staining of VAChT and p75NTR in Rat Medial Septum
Multiplex Staining of Plexin-A2 and p75NTR in Rat Brain
Multiplex Staining of Kir3.2 and KCNK2 in Rat Substantia Nigra
Multiplex Staining of VGAT and Gephyrin in Rat Spinal Cord
Multiplex Staining of Kir4.1 and α1-Syntrophin in Rat Fornix
Multiplex Staining of proNGF and Cannabinoid Receptor 1 in Mouse Hippocampus
Multiplex Staining of NET and NK1 Receptor in Rat Brain Stem
Multiplex Staining of GluR1 and PICK1 in Rat Hippocampus
Multiplex Staining of TRPM8 and TrkA in Rat DRG
Multiplex Staining of ASIC1 and ASIC3 in Rat DRG
Multiplex Staining of DPP6 and KV4.2 in Rat Hippocampus
Multiplex Staining of GluN2A and GluN2B in Mouse Deep Cerebellar Nucleus
Multiplex Staining of NaV1.8 and Synaptophysin in Rat DRG
Multiplex Staining of HCN4 and HCN2 in Mouse Thalamus
Multiplex Staining of KV1.3 and KV1.5 in Mouse Cerebellum
Multiplex Staining of TrkB and BDNF in Mouse Hippocampus
Multiplex Staining of GluN1 and Plexin-A1 in Rat Olfactory Bulb
Multiplex Staining of GluN1 and M4 Muscarinic Receptor in Mouse Parietal Cortex
Multiplex Staining of CaV1.2 and GABA(A) α1 Receptor in Rat Hippocampus
Multiplex Staining of TRPC6 and TRPC3 in Rat Cerebellum
Multiplex Staining of GluN1 and CALHM1 in Mouse Hippocampal CA1 Region
Multiplex Staining of ASIC1 and Parvalbumin in Rat Hippocampus
Multiplex Staining of Neurexin 1α and GABA(A) ε Receptor in Mouse Cerebellum
Multiplex Staining of mGluR1 and TRPC3 in Mouse Cerebellum
Multiplex Staining of PSD-95 and GluN2B in Rat Brain
Multiplex Staining of nAChR α4 and nAChR β2 in Mouse Brain
Multiplex Staining of VMAT2 and TRPC3 in Rat Brain
Multiplex Staining of AT2 Receptor and VGLUT2 in Rat Supraoptic Hypothalamic Nucleus
Multiplex Staining of Aquaporin 2 and Neutrophil Gelatinase-Associated Lipocalin (Ngal) in Mouse Kidney
Multiplex Staining of Aquaporin 2 and TRPV4 in Rat Kidney
Multiplex Staining of PTH2R and VGLUT2 in Mouse Hippocampus
Tityustoxin-Kα-ATTO Fluor-594 Colocalizes with KV1.2 Channel and Parvalbumin in Mouse Cerebellum
Multiplex Staining of p75NTR and TrkA in Rat DRG
Multiplex Staining of CaV1.2 and GABA(A) α1 Receptor in Mouse Hippocampus
Multiplex Staining of VGLUT2 and P2X7 Receptor in Rat Spinal Cord
Multiplex Staining of KCa2.3 (SK3) and Aquaporin 2 in Mouse Kidney
Multiplex Staining of KV1.1 and KV1.2 in Mouse Cerebellum
Multiplex Staining of CaV1.2 and GABA(A) α1 Receptor in Rat Cerebellum
Multiplex Staining of TrkB in Mouse Cerebellum
Multiplex Staining of Melatonin Receptor Type 1B and AT2 Receptor in Rat Brain
Multiplex Staining of Melatonin Receptor Type 1B and SCN2A (NaV1.2) in Rat Paraventricular Nucleus
Multiplex Staining of TrKA and NGF in Mouse Cerebellar Cortex
Multiplex Staining of KCa3.1 and TRPC1 in Mouse Brain
Multiplex Staining of Kir4.1 and GFAP in Rat Hippocampal Dentate Gyrus
Multiplex Staining of VGLUT2 and Neurokinin 1 Receptor in Rat DRG
Multiplex Staining of TRPV1 and NaV1.8 in Rat DRG
Multiplex Staining of TRPV1 and mGluR5 in Rat DRG
Multiplex Staining of NK1 Receptor and mGluR5 in Rat DRG
Multiplex Staining of TRPV2 and mGluR5 in Rat DRG
Multiplex Staining of Orexin Receptor 1 and Parathyroid Hormone 1 Receptor in Rat Ventromedial Hypothalamus
Multiplex Staining of GluR1 and Vesicular GABA Transporter in Human U-87 MG Cells
Multiplex Staining of TRPC6 and TRPC3 in Rat Cerebellum
Multiplex Staining of P2X7 Receptor and Iba-1 Microglia Marker in Mouse Spinal Cord
Multiplex Staining of CaV1.2 and GABA(A) α1 Receptor in Rat Cerebellum