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Anti-µ-Opioid Receptor (OPRM1) (extracellular)-ATTO Fluor-488 Antibody

Mu-type opioid receptor, Opioid receptor B, MOR-1, MOP, Mu-opiate receptor

Cat #: AOR-011-AG
Alternative Name Mu-type opioid receptor, Opioid receptor B, MOR-1, MOP, Mu-opiate receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CSPAPGSWLNLSHVDGN, corresponding to amino acid residues 22-38 of rat µ-Opioid receptor (Accession P33535). Extracellular, N-terminus.
Accession (Uniprot) Number P33535
Gene ID 25601
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 15/17 amino acid residues identical.
RRID AB_2341025.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AOR-011), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: ic*, ih*, lci*
Direct flow cytometry
  • Cell surface detection of OPRM1 in intact living human T-lymphocyte (Jurkat) cells:
    Cell surface detection of OPRM1 in intact living human T-lymphocyte (Jurkat) cells:
    ___ Unstained cells.
    ___ Cells + Anti-µ-Opioid Receptor (OPRM1) (extracellular)-ATTO Fluor-488 Antibody (#AOR-011-AG), (10-15 µg/5x105 cells).
References
  1. Pan, L. et al. (2005) Neuroscience 133, 209.
  2. http://www.guidetopharmacology.org/.
  3. Matthes, H.W. et al. (1998) J. Neurosci. 18, 7285.
  4. Shaqura, M.A. et al. (2004) J. Pharmacol. Exp. Ther. 308, 712.
  5. Baraldi, P.G. et al. (2006) Curr. Med. Chem. 13, 3467.
Scientific background

Endogenous opiates such as endorphins, endomorphins, and enkephalins, as well as opiate drugs (including morphine) exert their effects by binding to opioid receptors. Three "classic" types of opioid receptors have been identified: mu (µ)-opioid (MOP) receptor, delta (δ)-opioid (DOP) receptor, and kappa (κ)-opioid (KOP) receptor.1 Recently, the nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptor was also described. Despite its significant sequence homology, its pharmacological profile differs greatly from those of the classic µ, δ, and κ receptors.2

The opioid receptors belong to the G protein-coupled receptor (GPCR) superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important for binding G proteins.1

All three receptors mediate opioid-induced analgesia. Supraspinal analgesia is mainly mediated by the µ-receptors, whereas µ-, δ-, and κ-receptors participate in the control of pain at the spinal level. These receptors also mediate the mood-altering properties of opioids.3

Of the opioid receptors, the µ-opioid receptor has been the most extensively studied due to its important role in mediating the actions of morphine and other analgesic agents, as well as other addictive drugs such as heroin.1 The µ-opioid receptors are expressed in the central nervous system (CNS) and in the peripherial nervous system.4  The highest densities are found in the thalamus, caudate putamen, neocortex, amygdala, and other brain regions known to have well established roles in pain and analgesia.5

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody (#AOR-011) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize MOR-1 from rat, human, and mouse samples.

Anti-µ-Opioid Receptor (OPRM1) (extracellular)-ATTO Fluor-488 Antibody (#AOR-011-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-µ-Opioid Receptor (OPRM1) (extracellular)-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

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