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Anti-5HT4 Receptor (HTR4) (extracellular) Antibody

5-Hydroxytryptamine receptor 4, Serotonin receptor 4

Cat #: ASR-036
Alternative Name 5-Hydroxytryptamine receptor 4, Serotonin receptor 4
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide (C)DVIEKRKFNHNSN, corresponding to amino acid residues 168-180 of rat 5-Hydroxytryptamine receptor 4 (Accession Q62758). 2nd extracellular loop.
    Accession (Uniprot) Number Q62758
    Gene ID 25324
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.
    RRID AB_2341076.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ifc, lci, wb
    May also work in: ih*, ip*
    Western blot
    • Western blot analysis of rat brain lysate:
      Western blot analysis of rat brain lysate:
      1. Anti-5HT4 Receptor (HTR4) (extracellular) Antibody (#ASR-036), (1:200).
      2. Anti-5HT4 Receptor (HTR4) (extracellular) Antibody, preincubated with 5HT4 Receptor/HTR4 (extracellular) Blocking Peptide (#BLP-SR036).
    Indirect flow cytometry
    • Cell surface detection of Serotonin receptor 4 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
      Cell surface detection of Serotonin receptor 4 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-5HT4 Receptor (HTR4) (extracellular) Antibody, (#ASR-036), (2.5μg) + goat-anti-rabbit-FITC.
    Live cell imaging / Immunocytochemistry
    • Expression of Serotonin receptor 4 in rat PC12 cells
      Expression of Serotonin receptor 4 in rat PC12 cells
      Cell surface detection of Serotonin receptor 4 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-5HT4 Receptor (HTR4) (extracellular) Antibody, (#ASR-036), (1:100), (red). B. Merge of A with a live view of the cell.
    1. Mnie-Filali, O. et al. (2010) Cell. Signal. 22, 501.
    2. Barnes, N.M. and Sharp, T. (1999) Neuropharmacology 38, 1083.
    3. Oh, D.Y. et al. (2006) Int. Rev. Cytol. 252, 163.
    4. Bockaert, J. et al. (2004) Curr. Drug Targets CNS Neurol. Disord. 3, 39.
    5. Varnas, K. et al. (2003) Eur. Neuropsychopharmacol. 13, 228.
    Scientific background

    5-hydroxytryptamine receptor 4 (5-HT4 receptors) are a subclass among the large family of receptors mediating serotonin actions at central and peripheral targets. 5-HT4 receptor activation has been implicated in learning and memory, feeding control and stress response. 5-HT4 receptors are encoded by a complex gene which in humans, generates ten different splice variants1, 2.

    The 5-HT4 receptor, a member of the seven transmembrane spanning G-protein-coupled receptors (GPCRs), couples via Gs to increase cAMP production. GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs); the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side3.

    Within the central nervous system, these receptors are expressed in limbic structures including: hypothalamus, nucleus accumbens, prefrontal cortex, amygdala and hippocampus, where they contribute to emotional and cognitive functions4.

    Low levels of 5-HT4 receptors are associated with numerous neuropsychiatric disorders including Alzheimer's disease, Huntington’s disease, feeding disorders and stress-related disorders such as anxiety and depression5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 08/01/2023

    Anti-5HT4 Receptor (HTR4) (extracellular) Antibody (#ASR-036) is a highly specific antibody directed against an epitope of the rat Serotonin receptor 4. The antibody can be used in western blot, immunocytochemistry, and live cell imaging applications.  It has been designed to recognize 5HT4 receptor from rat, mouse, and human samples.

    For research purposes only, not for human use



    KO validation citations
    1. Immunohistochemstry and immunocytochemistry of mouse brain and hippocampal cultured astrocytes. Tested in 5-HT4 Receptor -/- mice.
      Müller, F.C. et al. (2021) Glia. 69, 872.


    Scientific Background

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