Anti-CD56/NCAM1 (extracellular)-ATTO-488 Antibody

NCAM-1, CD56
  • New
Cat #: ANR-041-AG
Alternative Name NCAM-1, CD56
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)DEKHIFSDDSSELTIR, corresponding to amino acid residues 261-276 of rat CD56 (NCAM1) (Accession P13596). Extracellular, N-terminus.
    • Anti-CD56/NCAM1 (extracellular)-ATTO-488 Antibody
    Accession (Uniprot) Number P13596
    Gene ID 24586
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical; human – 13/15 amino acids residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANR-041), and immunohistochemical staining (labeled antibody).
    Applications: ih
    May also work in: ic*, lci*
    Immunohistochemistry
    • Anti-CD56/NCAM1 (extracellular)-ATTO-488 Antibody
      Expression of CD56 in rat brain
      Immunohistochemical staining of rat olfactory bulb free-floating frozen sections using Anti-CD56/NCAM1 (extracellular)-ATTO-488 Antibody (#ANR-041-AG), (1:60). CD56 immunoreactivity (green) appears in glomerular structures (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).
    References
    1. Johnson, C.P. et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101, 6963.
    2. Lanier, L.L. et al. (1991) J. Immunol146, 4421.
    3. Kiss, J.Z. and Muller, D. (2001) Rev. Neurosci12, 297.
    4. Arai, M. et al. (2004) Biol. Psychiatry 55, 804.
    5. Ćirović, S. et al. (2014) Int. J. Clin. Exp. Pathol7, 1402.
    Scientific background

    Neural cell adhesion molecule 1 (NCAM-1) is a member of a large family of cell-surface glycoproteins and plays a major role during development and controls various functions in the nervous system such as cell migration, neurite growth, axonal guidance, and synaptic plasticity1. NCAM-1 mediates adhesion between cells through homophilic bonds formed between its extracellular domains, which comprise five tandem Ig domains and two juxtamembrane fibronectin type III (Fn III) domains2. There are three major protein isoforms of NCAM-1: 180 kd, 140 kd, and 120 kd. The 180-kd and 140-kd isoforms of NCAM-1 are transmembrane proteins, whereas the 120-kd isoform is linked to the plasma membrane via a glycosyl phosphatidyl-inositol (GPI) lipid anchor3

    NCAM-1 is widely expressed in the central nervous system, skeletal muscle and natural killer cells4.

    Several lines of evidence have supported the idea that dysregulation of NCAM-1 isoforms in the brain might be involved in the pathophysiology of neuropsychiatric disorders, particularly bipolar affective disorder5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 27/02/2020

    Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell imaging, and immunohistochemistry applications. It has been designed to recognize CD56 from rat, mouse and human samples.

    Anti-CD56/NCAM1 (extracellular)-ATTO-488 Antibody (#ANR-041-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-CD56/NCAM1 (extracellular)-ATTO-488 Antibody is suited for experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use
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