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Anti-Connexin-32 Antibody

CX32, Gap junction β1 protein, GJB1, CMTX

Cat #: ACC-211
Alternative Name CX32, Gap junction β1 protein, GJB1, CMTX
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EINKLLSEQDGSLK, corresponding to amino acid residues 247-260 of rat Connexin-32 (Accession P08033). Intracellular, C-terminus.
Accession (Uniprot) Number P08033
Gene ID 29584
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human, mouse – identical.
RRID AB_11218955.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat liver lysate:
    Western blot analysis of rat liver lysate:
    1. Anti-Connexin-32 Antibody (#ACC-211), (1:200).
    2. Anti-Connexin-32 Antibody, preincubated with Connexin-32 Blocking Peptide (#BLP-CC211).
References
  1. Goodenough, D.A. and Paul, D.L. (2011) Cold Spring Harb. Persp. Biol. 2009, 1.
  2. Nakagawa, S. et al. (2010) Curr. Opin. Struct. Biol. 20, 423.
  3. Chew, S.S. et al. (2010) Exp. Neurol. 225, 250.
  4. Trexler, E.B. et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 5836.
  5. Hu, X. et al. (2006) Biophys. J. 90, 140.
  6. Tang, Q. et al. (2009) J. Gen. Physiol. 133, 555.
  7. Kleopa, K.A. (2011) J. Neurosci. 31, 17753.
Scientific background Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels1,2. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications1.

Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells3. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2,4,5.

Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases and developmental abnormalities2,5,6. A mutation in the gene encoding Connexin-32 is associated with and is the cause for Charcot–Marie–Tooth disease (CMT), a form of demyelinating disease characterized by muscular weakness, affecting more males than females7.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 25/07/2021

Anti-Connexin-32 Antibody (#ACC-211) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Cx32 from human, rat, and mouse samples.

For research purposes only, not for human use
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