Anti-Connexin-45 Antibody

CX45, Gap junction γ1 protein, GJC1, Gap junction α7 protein, GJA7
Cat #: ACC-207
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)KEQSQPKPKHDGRR, corresponding to amino acid residues 153-166 of rat Connexin-45 (Accession A4GG66). Intracellular loop.
    Accession (Uniprot) Number A4GG66
    Gene ID 266706
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
    Western blot
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1,2. Anti-Connexin-45 Antibody (#ACC-207), (1:200).
    3,4. Anti-Connexin-45 Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:
    1. Anti-Connexin-45 Antibody (#ACC-207), (1:200).
    2. Anti-Connexin-45 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of Connexin-45 in rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Connexin-45 Antibody (#ACC-207), (1:400), followed by anti-rabbit-Cy3 antibody. Cx45 staining (red) appears in the neuropil of layers stratum oriens and stratum radiatum but not in the pyramidal layer. Nuclei are stained with DAPI (blue).
    References
    1. Goodenough, D.A. and Paul, D.L. (2009) Cold Spring Harb. Perspect. Biol. 1, a002576.
    2. Nishii, K. et al. (2014) World J. Stem Cells 6, 571.
    3. Sirnes, S. et al. (2011) Epigenetics 6, 6023.
    4. Sohl, G. et al. (2004) Cardiovasc Res. 62, 228.
    5. Fang, J.S. et al. (2013) Arterioscler. Thromb. Vasc. Biol. 33, 362.
    6. Salameh, A. et al. (2013) Front. Pharmacol. 4, 706.
    Scientific background

    Gap junctions are plasma membrane domains of intercellular channels that allow direct connection between the cytoplasm of adjacent cells. They allow exchange of low molecular weight metabolites, ions and small hydrophilic molecules. These channels are formed by head-to-head docking of hexameric hemichannels called connexons, whose subunit proteins are encoded by the connexin (Cx) gene family1-3.

    The connexin subunit is formed of four-transmembrane spanning domains, harboring two extracellular loops, one cytoplasmic loop and cytoplasmic N- and C-terminal regions. To date, the family of connexin comprises 21 genes in the human genome4.

    Connexin 45 (Cx45), encoded by the GJC1 gene, is expressed in cardiomyocytes and in developing vasculature in both endothelial and smooth muscle cells4,5.

    In the heart, Cx45 along with Cx40 and Cx43, play a crucial role in normal electrical conduction and synchronous heart muscle contraction and important for the normal embryonic development of the heart6. Indeed Cx45 forms low conductance and voltage-sensitive gap junctions between the ventricular cardiac myocytes and the core conduction system2

    Studies have showed that cardiac connexin Cx45 is upregulated in human failing hearts and has an important role in the regulation of endothelial-induced mural cell differentiation4,5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 11/03/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Connexin-45. Anti-Connexin-45 Antibody (#ACC-207) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Connexin-45 from human, rat and mouse samples.

    For research purposes only, not for human use