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Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody

EMR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480

Cat #: AER-051-F
Alternative Name EMR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1 (Accession Q14246). Extracellular, N-terminus.
Accession (Uniprot) Number Q14246
Gene ID 2015
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, Rat –11/14 amino acid residues identical.
RRID AB_2756589.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label Fluorescein isothiocyanate (FITC).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AER-051), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: ic*, lci*
Direct flow cytometry
  • Cell surface detection of EMR1 in live intact mouse J774 macrophage cells:
    Cell surface detection of EMR1 in live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody (#AER-051-F), (2.5 µg).
  • Cell surface detection of EMR1 in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of EMR1 in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody (#AER-051-F), (2.5 µg).
References
  1. Legrand, F. et al. (2014) J. Allergy Clin. Immunol. 133, 1439.
  2. McKnight, A.J. and Gordon, S. (1998) J. Leukoc. Biol. 63, 271.
  3. Araç, D. et al. (2012) EMBO J. 31, 1364.
Scientific background

Human epidermal growth factor (EGF)–like module containing mucin-like hormone receptor 1 (EMR1) is a surface receptor with unknown function that belongs to the adhesion family of the G protein-coupled receptors (GPCR). This receptor is encoded by the ADGRE1 and like all GPCRs, contains seven transmembrane domains, an intracellular C-terminus and an extracellular N-terminus. The NH2 domain has six EGF-like domains.

Adhesion GPCRs share a long N-terminal extracellular sequences comprising multiple domains, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties1,2.

EMR1 protein is a homolog of F4/80, a murine protein that is widely being used as a marker of murine macrophage populations1.

Human EMR1 is exclusively expressed on mature eosinophils in the blood and bone marrow and in nasal polyps. Thus, EMR1 is considered to be a potential therapeutic target for the treatment of eosinophilic disorders1,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell imaging applications. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize EMR1 from human, mouse, and rat samples.

Anti-EMR1 (ADGRE1) (extracellular)FITC Antibody (#AER-051-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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