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Anti-GABA(B) Receptor 1 (extracellular)-FITC Antibody

γ-Aminobutyric acid type B receptor subunit 1, GABA B receptor 1, GABABR1, Gb1, GABBR1, GPRC3A

Cat #: AGB-001-F
Alternative Name γ-Aminobutyric acid type B receptor subunit 1, GABA B receptor 1, GABABR1, Gb1, GABBR1, GPRC3A
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CRGEREVVGPKVRK, corresponding to amino acid residues 66-79 of rat GABBR1 (Accession Q9Z0U4). Extracellular, N-terminus.
Accession (Uniprot) Number Q9Z0U4
Gene ID 81657
Homology Mouse, human, canine, bovine - identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGB-001), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
  • Cell surface detection of GABA(B) Receptor 1 by direct flow cytometry in live intact mouse BV-2 microglia cells:
    Cell surface detection of GABA(B) Receptor 1 by direct flow cytometry in live intact mouse BV-2 microglia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-GABA(B) Receptor 1 (extracellular)-FITC Antibody (#AGB-001-F), (2.5µg).
  • Cell surface detection of GABA(B) Receptor 1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of GABA(B) Receptor 1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-GABA(B) Receptor 1 (extracellular)-FITC Antibody (#AGB-001-F), (5µg).
References
  1. Bowery, N.G. and Smart, T.G. (2006) Br. J. Pharmacol. 147, S109.
  2. Bettler, B. et al. (2004) Physiol. Rev. 84, 835.
  3. Boller, M. and Schmidt, M. (2003) J. Neurophysiol. 89, 2035.
  4. Robbins, M.J. et al. (2001) J. Neurosci. 21, 8043.
  5. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 14649.
  6. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 14643.
  7. https://www.uniprot.org/uniprot/Q9Z0U4
Scientific background

GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2

The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA(A) R and GABA(C) R, and the metabotropic GABA (B) receptor, [GABA(B) R].3 The latter belongs to the G-protein coupled receptor superfamily and mediates slow synaptic inhibition in the brain and spinal cord.1,4

The functional GABA (B) receptor is a heterodimer consisting of two subunits, the GABA(B) R1 and the GABA(B) R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6 The GABA(B) R1 subunit was demonstrated to be important for agonist and antagonist binding, while GABA(B) R2 was shown to be essential for trafficking and for G-protein binding.

To date, eight alternatively spliced isoforms of GABA (B) R1 have been proposed. These are named 1a-1h, of which 1a and 1b are the most prominent. Only 1a, 1b, and 1c appear to act as functional subunits.6,7 They are widespread, being expressed in the testis, stomach, spinal cord, and brain. Isoform 1b is also expressed in the kidney and liver.7

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/01/2022

Anti-GABA(B) Receptor 1 (extracellular) Antibody (#AGB-001) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize GABBR1 from mouse, rat and human samples.

Anti-GABA(B) Receptor 1 (extracellular)-FITC Antibody (#AGB-001-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize GABBR1 from mouse, rat and human samples.

For research purposes only, not for human use
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