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Anti-GFR alpha 1 (extracellular) Antibody

GFRA1, GFRα1, GDNF family receptor α1, GDNF receptor alpha 1, Ret ligand 1, RETL1, TRNR1

Cat #: ANT-021
Alternative Name GFRA1, GFRα1, GDNF family receptor α1, GDNF receptor alpha 1, Ret ligand 1, RETL1, TRNR1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CRSAMEALKQKS, corresponding to amino acid residues 72-83 of rat GFRA1 (Accession Q62997). Extracellular domain.
Accession (Uniprot) Number Q62997
Gene ID 25454
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_10918530.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-GFR alpha 1 (extracellular) Antibody (#ANT-021), (1:200).
    3,4. Anti-GFR alpha 1 (extracellular) Antibody, preincubated with GFR α 1 (extracellular) Blocking Peptide (#BLP-NT021).
Immunohistochemistry
  • Expression of GFRA1 in rat neocortex
    Expression of GFRA1 in rat neocortex
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GFR alpha 1 (extracellular) Antibody (#ANT-021), (1:100). A. GFRA1 (green) is visualized in neocortex neurons. B. Neurons expressing γ-amino butyric acid (GABA) are labeled with parvalbumin (red). C. Merge of the two images demonstrates partial colocalization (arrows).
Live cell imaging / Immunocytochemistry
  • Expression of GFRA1 in live intact rat C6 glioma cells
    Expression of GFRA1 in live intact rat C6 glioma cells
    Cell surface detection of GFRA1 in live intact rat C6 glioma cells with Anti-GFR alpha 1 (extracellular) Antibody (#ANT-021), (1:50), followed by goat-anti-rabbit-DyLight-594 secondary antibody (red) (A). B. Live view of the cells. C. Merge of the two images.
References
  1. Airaksinen, M.S. and Saarma, M. (2002) Nat. Rev. Neurosci. 3, 383.
  2. Paratcha, G. and Ledola, F. (2008) Trends Neurosci. 31, 384.
  3. Paratcha, G. et al. (2001) Neuron 29, 171.
Scientific background

The GDNF family ligands (GFLs) belong to the super family of the TGF-β. They belong to the group of cystine-knot protein and function as homodimers1. This family includes glial cell line-derived neurotrophic factor (GDNF), artemin (ARTN), Neurturin (NRTN) and persephin (PSPN)2.

These factors are heavily involved in the development and function of the nervous system (both central and peripheral). In particular GDNF has an important role outside the nervous system where it plays a role in kidney morphogenesis1.

In general GFLs all signal through a signal through the receptor tyrosine kinase Ret. Their specificity is implemented by different GDNF family receptor a (GFRα), which act as co-receptors. These extracellular proteins are bound to the plasma membrane via a glycosyl phosphatidyl inositol (GPI) anchor. GFRα1-4 are responsible for the binding of GDNF, NRTN, ARTN, and PSPN respectively and the subsequent activation of Ret. Soluble forms of the receptor by the cleavage of a yet unknown phospholipase or protease can be detected1,3. Also alternative spliced forms of the protein can lead to soluble forms of GFRα receptors3.

In respect to their important role in development, individual knockout of either gdnf, gfra1 or ret gives rise to lethal phenotypes2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GDNF receptor α1. Anti-GFR alpha 1 (extracellular) Antibody (#ANT-021) can be used in western blot, immunohistochemistry, immunocytochemistry and live cell imaging applications. It has been designed to recognize GFR α1 from mouse, rat and human samples.

For research purposes only, not for human use
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