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Anti-GPR183 (extracellular) Antibody

G-Protein Coupled Receptor 183, Epstein-Barr Virus-Induced G-Protein Coupled Receptor 2, EBI2

Cat #: AGR-063
Alternative Name G-Protein Coupled Receptor 183, Epstein-Barr Virus-Induced G-Protein Coupled Receptor 2, EBI2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)GND(S)DLYAHHSTAR, corresponding to amino acid residues 18 - 31 of human GPR183 (Accession P32249). Extracellular, N-terminus.
Accession (Uniprot) Number Q9Z306
Gene ID 1880
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human – 13 out of 14 amino acid residues identical. Mouse, rat – 12 out of 14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain lysates (lanes 2 and 5) and rat lung membranes (lanes 3 and 6):
    Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain lysates (lanes 2 and 5) and rat lung membranes (lanes 3 and 6):
    1-3. Anti-GPR183 (extracellular) Antibody (#AGR-063), (1:200).
    4-6. Anti-GPR183 Antibody, preincubated with GPR183 (extracellular) Blocking Peptide (BLP-GR063).
  • Western blot analysis of rat spleen membranes (lanes 1 and 3) and mouse spleen membranes (lanes 2 and 4):
    Western blot analysis of rat spleen membranes (lanes 1 and 3) and mouse spleen membranes (lanes 2 and 4):
    1-2. Anti-GPR183 (extracellular) Antibody (#AGR-063), (1:200).
    3-4. Anti-GPR183 Antibody, preincubated with GPR183 (extracellular) Blocking Peptide (BLP-GR063).
  • Western blot analysis of human Daudi B-lymphoblast cell line lysates (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysates (lanes 2 and 4):
    Western blot analysis of human Daudi B-lymphoblast cell line lysates (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysates (lanes 2 and 4):
    1-2. Anti-GPR183 (extracellular) Antibody (#AGR-063), (1:200).
    3-4. Anti-GPR183 Antibody, preincubated with GPR183 (extracellular) Blocking Peptide (BLP-GR063).
Immunohistochemistry
  • Expression of GPR183 in in rat parietal cortex.
    Expression of GPR183 in in rat parietal cortex.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR183 (extracellular) Antibody (#AGR-063), (1:1200), followed by donkey anti-rabbit-biotin and streptavidin-Cy3. A. GPR183 immunoreactivity (red) appears in soma and apical dendrite profiles (arrows). B. Pre-incubation of the antibody with GPR183 (extracellular) Blocking Peptide (BLP-GR063), suppressed staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of GPR183 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of GPR183 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-GPR183 (extracellular) Antibody (#AGR-063), (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Kurschus FC, Wanke F. (2018), Biochimie., 153, 52.
  2. Daugvilaite et al (2014), Eur J Immunol., 44, 1904.
  3. Duc et al (2019), Int J Mol Sci., 20, 4522.
  4. Yi et al (2012), Immunity, 37, 535.
Scientific background

G-protein coupled receptor 183 (GPR183) also known as Epstein-Barr virus-induced gene 2 (EBI2), is a chemotactic receptor that participates in the migratory capability of cells 2. It belongs to the rhodopsin-like subfamily of class A transmembrane spanning (7TM) G protein-coupled receptors and its most potent ligand is oxysterol 7α-25-OHC 1.

Dihydroxycholesterols, such as oxysterol 7α-25-OHC, are generated from cholesterol in steady state but also in the context of inflammation. GPR183 senses these oxysterols and induces chemotactic migration of immune cells towards higher concentrations of these ligands and hence cells that express GPR183 are trafficking through an oxysterol gradient dependent manner acting like chemokine processes. The migratory function of this receptor affects several important immune processes, in particular, GPR183 is involved in the T-dependent antibody response in the germinal centers. This puts GPR183 in a unique position to effect autoimmunity and inflammatory bowel diseases 3,4.

In addition, GPR183 is highly expressed by type 3 innate lymphoid cells (ILC3) and its oxysterol ligand was shown to be essential for the localization and the migration of ILC3s and to have a critical role for the formation of lymphoid tissues in the mouse colon during colitis 2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-GPR183 (extracellular) Antibody (#AGR-063) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize GPR183 from mouse, rat and human samples.

For research purposes only, not for human use
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