Anti-GPR52 Antibody

G-Protein Coupled Receptor 52
  • New
Cat #: AGR-058
Alternative Name G-Protein Coupled Receptor 52
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)DRRARFPSHEVEASR, corresponding to amino acid residues 239 - 253 of mouse GPR52 (Accession P0C5J4). 3rd intracellular loop.
    • Anti-GPR52 Antibody
    Accession (Uniprot) Number P0C5J4
    Gene ID 620246
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat – 14/15 amino acid residues identical; human – 13/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    • Anti-GPR52 Antibody
      Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):
      1, 2. Anti-GPR52 Antibody (#AGR-058), (1:200).
      3, 4. Anti-GPR52 Antibody, preincubated with the negative control antigen.
    • Anti-GPR52 Antibody
      Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
      1. Anti-GPR52 Antibody (#AGR-058), (1:200).
      2. Anti-GPR52 Antibody, preincubated with the control peptide antigen.
    References
    1. Komatsu, H. et al. (2014) PLoS One 9, e90134.
    2. Setoh, M. et al. (2014) J. Med. Chem. 57, 5226.
    3. Lin, X. et al. (2020) Nature 579, 152.
    Scientific background

    G-Protein Coupled Receptor 52 (GPR52) is a conserved class-A orphan receptor, a member of the of the GPCR superfamily. The native ligand for GPR52 is unknown but the receptor has a high level of basal activity not related to ligand binding. Structure analysis shows that GPR52 extracellular loop 2 occupies the orthosteric binding pocket and operates as a built-in agonist, enabling an intrinsically high level of basal activity. The receptor also features a side pocket for ligand binding3.

    Tissue distribution analysis of human, mouse, and rat indicates that GPR52 is expressed exclusively in the brain, especially in striatum. In the human brain, GPR52 expression profile overlaps with the distribution of D1 and D2 dopamine receptors which are associated with its function1.

    GPR52 inhibits dopamine D2 receptor signaling and activates dopamine D1/NMDA receptors via intracellular cAMP accumulation2.

    GPR52 is involved in the manifestation of schizophrenia, Huntington’s disease, cognitive impairment and hyperactivity and several other psychiatric disorders and hence it represents a promising therapeutic target. Agonists may potentially act as a novel class of antipsychotics1,2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 27/07/2020

    Anti-GPR52 Antibody (#AGR-058) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize GPR52 from human, mouse, and rat samples.

    For research purposes only, not for human use
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