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Anti-Human CCR5 (extracellular) Antibody

C-C chemokine receptor type 5, C-C CKR-5, CHEMR13, HIV-1 fusion coreceptor, CD195

Cat #: ACR-025
Alternative Name C-C chemokine receptor type 5, C-C CKR-5, CHEMR13, HIV-1 fusion coreceptor, CD195
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide CSSHFPYSQYQFWKN, corresponding to amino acid residues 178-192 of human CCR5 (Accession P51681). 2nd extracellular loop.
Accession (Uniprot) Number P51681
Gene ID 1234
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human only.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended for use with rat and mouse samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, lci, wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of human HL-60 promyelocytic leukemia (lanes 1 and 4), human THP-1 acute monocytic leukemia (lanes 2 and 5) and human MEG-O1 Chronic myelogenous leukemia  (lanes 3 and 6) cell lysates:
    Western blot analysis of human HL-60 promyelocytic leukemia (lanes 1 and 4), human THP-1 acute monocytic leukemia (lanes 2 and 5) and human MEG-O1 Chronic myelogenous leukemia  (lanes 3 and 6) cell lysates:
    1-3. Anti-Human CCR5 (extracellular) Antibody (#ACR-025), (1:400).
    4-6. Anti-Human CCR5 (extracellular) Antibody, preincubated with Human CCR5 (extracellular) Blocking Peptide (#BLP-CR025).
Indirect flow cytometry
  • Cell surface detection of Human CCR5 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of Human CCR5 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells +  Anti-Human CCR5 (extracellular) Antibody (#ACR-025), (2.5μg) + goat-anti-rabbit-FITC .
References
  1. Colin, P. et al. (2018) PLoS Pathog. 14, e1007432.
  2. Ni, J. et al. (2018) Open Med. (Wars) 13, 467.
  3. Hoover, K.C. et al. (20180 PLoS One 13, e0204989.
Scientific background

CCR5 protein is a G-protein coupled receptor (GPCR) responsible for regulating host immune responses and functions against pathogens. The protein plays a key role in transferring immune cells to inflammatory sites and injury. CCR5 is also considered to be an anchor protein for R5-tropic strains of HIV-1 (Human Immunodeficiency Virus-1) to infect immune cells. Thus, CCR5 contributes to the development of AIDS (Acquired Immunodeficiency Syndrome).

The receptor is widely expressed in a variety of immune cells involved in the inflammatory responses such as macrophages, monocytes, T cells and dendritic cells1,2.

CCR5 is the receptor for the CC ligand 3 (CCL3), CC ligand 4 (CCL4), and CC ligand 5 (CCL5) chemokine ligands. CCR5 and its ligands are upregulated during HIV infection.

Studies have shown that lack of homozygosity of CCR5Δ32 variant, a 32 base-pair deletion of the CCR5’s gene coding region, is associated with preventing the risk of HIV-1 infection2,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-Human CCR5 (extracellular) Antibody (#ACR-025) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize CCR5 from human samples only. The antibody is unlikely to recognize the receptor from mouse and rat samples.

For research purposes only, not for human use
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