Anti-p75 NGF Receptor (intracellular) Antibody

p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271
    Cat #: ANT-011
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide CKQNKQGANSRPVNQT corresponding to amino acid residues 278-293 of human p75NTR (Accession P08138). Intracellular, juxtamembrane domain.
    Accession (Uniprot) Number P08138
    Gene ID 4804
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
    Western blot
    Western blot analysis of rat brain membranes:
    1. Anti-p75 NGF Receptor (intracellular) Antibody (#ANT-011), (1:200).
    2. Anti-p75 NGF Receptor (intracellular) Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human melanoma cells A875:
    1. Anti-p75 NGF Receptor (intracellular) Antibody (#ANT-011), (1:200).
    2. Anti-p75 NGF Receptor (intracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of p75NTR in mouse hippocampus
    Immunohistochemical staining of mouse frozen hippocampal sections using Anti-p75 NGF Receptor (intracellular) Antibody (#ANT-011) (1:200). A. p75NTR (green) appears in a subset of astrocytes. GFAP (glial fibrillary acidic protein) staining (red) to label astrocytes. C. A merge of the two images shows p75NTR found mainly in stratum radiatum (SOR) and only in a few astrocytes in the alveus (AL).
    References
    1. Hempstead, B.L. (2002) Curr. Opin. Neurobiol. 12, 260.
    2. Chao, M.V. (2003) Nat. Rev. Neurosci4, 299.
    3. Barker, P.A. (2004) Neuron 42, 529.
    Scientific background

    The p75 neurotrophin receptor (p75NTR) is a member of the TNF receptor superfamily. p75NTR, like all the superfamily members is a type I transmembrane protein with tandem cysteine-rich domains in the extracellular portion and a intracellular death domain. p75NTR binds its ligands as a homodimer but can also form heterodimers with other receptors such as TrkATrkBTrkCNogo receptor and sortilin. The precise multimeric receptor complex formed between p75NTR and the other receptors will determine the ligand being recognized (see below) and the biological response to its binding.

    As it name implies, p75NTR binds all the neurotrophins (NGFBDNFNT-3 and NT-4) with similar nM affinities. Co-expression of p75NTR with the Trk receptors enhances the ability to bind and respond to the specific neurotrophin and induces cell survival. On the other hand, it has recently been demonstrated that p75NTR binds with high affinity to the unprocessed form of NGF (proNGF) probably as a complex with sortilin and this leads to cell death by apoptosis. Finally, a multimeric complex of p75NTR and Nogo receptor binds myelin proteins such as Nogo-66, MAG and OmgP resulting in inhibition of axonal growth.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/12/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human p75NTR. Anti-p75 NGF Receptor (intracellular) Antibody (#ANT-011) can be used in western blot and immunohistochemistry applications. It has been designed to recognize p75NTR from human, mouse and rat samples.

    For research purposes only, not for human use
    Citations
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