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Anti-MCT4 (SLC16A3) Antibody

Monocarboxylate transporter 4, Monocarboxylic acid transporter 4, MOT4

Cat #: AMT-014
Alternative Name Monocarboxylate transporter 4, Monocarboxylic acid transporter 4, MOT4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)REVEHFLKAEPEK, corresponding to amino acid residues 447-459 of rat MCT4 (Accession O35910). Intracellular, C-terminus.
Accession (Uniprot) Number O35910
Gene ID 80878
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756672.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-MCT4 (SLC16A3) Antibody (#AMT-014), (1:200).
    3,4. Anti-MCT4 (SLC16A3) Antibody, preincubated with MCT4/SLC16A3 Blocking Peptide (#BLP-MT014).
  • Western blot analysis of human K562 chronic myelogenous leukemia cell line lysate (lanes 1 and 3) and human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 2 and 4):
    Western blot analysis of human K562 chronic myelogenous leukemia cell line lysate (lanes 1 and 3) and human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 2 and 4):
    1,2. Anti-MCT4 (SLC16A3) Antibody (#AMT-014), (1:200).
    3,4. Anti-MCT4 (SLC16A3) Antibody, preincubated with MCT4/SLC16A3 Blocking Peptide (#BLP-MT014).
References
  1. Yan, P. et al. (2014) Asian Pac. J. Cancer Prev. 15, 8923.
  2. Luz, M.C. et al. (2017) Int. J. Mol. Sci. 18, 170.
  3. Noor, S.I. et al. (2017) FEBS J. 284, 149.
  4. Nakayama, Y. et al. (2012) Exp. Ther. Med. 3, 25.
Scientific background

Monocarboxylate transporter 4 (MCT4) belongs to a group of membrane proteins responsible for mediating the transfer of various short chain monocarboxylates including lactate, pyruvate, and ketones across the cell membrane. MCTs also transfer H+ resulting in acidification of the tissue microenvironment, thus playing an important role in the control of intracellular pH by proton‐coupled release. MCT4 transports monocarboxylates in a ratio of 1:1 with H+.

Transport activity of MCT4 is enhanced by carbonic anhydrase II, which has been suggested to function as a “proton antenna” for MCT41-3.

The SLC16 gene family of MCTs comprises 14 members with a structure that contains 12 transmembrane domains. Among MCT members, only MCT1 and MCT4 catalyze the proton-coupled transport of lactate and H+. The distribution of MCTs is different by cell type. MCT4 is highly expressed in glycolytic cells, such as skeletal muscle fibers, astrocytes, leukocytes, chondrocytes, and some mammalian cell lineages.

MCT4 is extensively investigated in cancer cells and is considered to be a metabolic target for anticancer therapies2-4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-MCT4 (SLC16A3) Antibody (#AMT-014) is a highly specific antibody directed against an epitope of the rat Monocarboxylate transporter 4. The antibody can be used in western blot analysis. It has been designed to recognize MCT4 from human, rat, and mouse samples.

For research purposes only, not for human use
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