- Peptide CEQRLDAIHSEVSMTFKAK, corresponding to amino acid residues 346-364 of mouse NPY2R (Accession P97295). Intracellular, C-terminus.
- Western blot analysis of rat hippocampus (lanes 1 and 3) and rat whole brain lysates (lanes 2 and 4):1,2. Anti-NPY2R Antibody (#ANR-022), (1:200).
3,4. Anti-NPY2R Antibody, preincubated with NPY2R Blocking Peptide (#BLP-NR022).
- Expression of neuropeptide Y2 receptor in rat cerebellumImmunohistochemical staining of frozen section of rat cerebellum using Anti-NPY2R Antibody (#ANR-022), (1:100). NPY2R (red) is detected in Purkinje cells. B. Nuclei were stained using DAPI as the counterstain. C. Merged image of A and B.
- Expression of neuropeptide Y2 receptor in rat dorsal root ganglion cellsImmunocytochemical staining of rat dorsal root ganglion (DRG) neurons. A, D. A paraformaldehyde-fixed and permeabilized DRG primary culture was stained with Anti-NPY2R Antibody (#ANR-022), (1:100) followed by Alexa-555-conjugated goat anti-rabbit secondary antibody. B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. F. Merged images of panels D and E.
- Stanic, D. et al. (2006) J. Comp. Neurol. 499, 357.
- Körner, M. et al. (2004) Clin. Cancer Res. 10, 8426.
- Sun, L. and Miller, R.J. (1999) J. Neurophysiol. 81, 1391.
- Bournat, J.C. and Allen, J.M. (2001) Brain Res. Mol. Brain Res. 90, 149.
- Wheway, J. et al. (2005) J. Exp. Med. 202, 1527.
- Silva, A.P. et al. (2006) FASEB J. 21, 671.
- Batterham, R.L. et al. (2003) N. Engl. J. Med. 349, 941.
Neuropeptide Y (NPY) is a 36-amino acid peptide neurotransmitter in the central (CNS) and peripheral nervous systems.1 It belongs to the NPY family, together with peptide YY (PYY) and pancreatic polypeptide (PP). NPY is involved in regulation of a broad range of homeostatic functions such as eating behavior, hypertension, and reproduction.2
NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these, Y1, Y2, Y4, and Y5, are expressed in humans.2
The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.
Activation of these receptors by NPY produces effects on [Ca2+]i, adenylate cyclase, and a number of ion channels.3 Based on the high frequency and density of NPY receptors in steroid hormone-producing tumors, the NPY receptors might be of potential use in tumor management.2
NPY2R is primarily expressed in neurons as a presynaptic receptor. It is also strongly expressed in epithelia of the visceral tissues, including colon and kidney.4 NPY2R is involved in inhibition of neurotransmitter release mediated by NPY.5 Release of glutamate, the principal neuroexcitatory transmitter, is inhibited via the NPY2R receptor.6 Involvement of NPY2R in feeding behavior was also reported. PYY(3–36), an NPY2R agonist, acts to inhibit feeding via NPY2R.7
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse neuropeptide Y2 receptor (NPY2R). Anti-NPY2R Antibody (#ANR-022) can be used in western blot analysis, immunohistochemistry, and immunocytochemical applications. It has been designed to recognize NPY2R from human, rat, and mouse samples.