Anti-Neuropilin-2 (extracellular)-FITC Antibody

Vascular Endothelial Cell Growth Factor 165 Receptor 2, VEGF165R2, NRP2
    Cat #: ANR-062-F
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)REDQGGEWKHGR, corresponding to amino acid residues 747 - 758 of human Neuropilin-2 (Accession O60462). Extracellular, N-terminus.
    Accession (Uniprot) Number O60462
    Gene ID 8828
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse – 11/12 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANR-062), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic, lci
    Direct flow cytometry
    Direct flow cytometry of live intact human THP-1 acute monocytic leukemia cell line:

    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Neuropilin-2 (extracellular)-FITC Antibody (ANR-062-F), (2.5 µg).

    References
    1. Wild, J.R. et al. (2012) Int. J. Exp. Pathol. 93, 81.
    2. Prud'homme, G.J. and Glinka, Y. (2012) Oncotarget. 3, 921.
    3. Bagri, A. et al. (2009) Clin. Cancer Res. 15, 1860.
    Scientific background

    The neuropilins (Nrps) are single-pass multifunctional transmembrane glycoproteins that play an important role in cell development, immunity and cancer, physiological and pathological angiogenesis. They were first discovered as regulators of nervous system development, acting as semaphorin coreceptors with plexins and also enhance cellular responses to members of the vascular endothelial growth factor (VEGF) family.

    They comprise of two members: Neuropilin-1 (NRP1) and Neuropilin-2 (NRP2) which are usually expressed as homodimers but also can appear as heterodimers. NRP2 contains a large N-terminal extracellular consisting of five domains, a short transmembrane domain and a small cytoplasmic domain1-3

    Nrp2 is expressed in a wide variety of cells including endothelial cells, neurons, pancreatic islet cells, hepatocytes, melanocytes and osteoblasts. Nrp2 has been shown to mediate proliferation, survival, and migration of tumor cells3.

    Nrp2 knockout mice studies show that mice are viable without defects in blood vessels but display abnormal lymphatic development and defects of the central and peripheral nervous system2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 22/10/2018

    Anti-Neuropilin-2 (extracellular) Antibody (#ANR-062) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize NRP2 from mouse, rat, and human samples.

    Anti-Neuropilin-2 (extracellular)-FITC Antibody (#ANR-062-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use