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Anti-Neurotrophin 4 (NT-4) Antibody

NTF4, Neutrophic factor 4, Neurotrophin 5, NT-5, NTF5, Neutrophic factor 5, NT 4/5

Cat #: ANT-004
Alternative Name NTF4, Neutrophic factor 4, Neurotrophin 5, NT-5, NTF5, Neutrophic factor 5, NT 4/5
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide LGEVPAAGGSPL(C), corresponding to amino acid residues 43-52 of mature human NT-4 (residues 123-132 of the precursor NT-4) (Accession P34130).
Accession (Uniprot) Number P34130
Gene ID 16485
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical.
RRID AB_2040017.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.3 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
Immunohistochemistry
  • Expression of Neurotrophin 4 in mouse spinal cord
    Expression of Neurotrophin 4 in mouse spinal cord
    Immunohistochemical staining of mouse spinal cord using Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004). The spinal ventral horn (VH) was very lightly stained. However, in the perimeter of the white matter (WM) there were NT-4 immunoreactive cells with a small soma (triangles) and some had a stained process (arrows).
References
  1. Roux, P. et al. (2002) Prog. Neurobiol. 67, 203.
  2. Roosen, A. et al. (2001) J. Neurosci. 21, 3073.
  3. Moalem, G. et al. (2002) J. Autoimmun. 15, 331.
  4. Marconi, A. et al. (2003) J. Invest. Dermatol. 121, 1515.
  5. McDonald, N.Q. et al. (1991) Nature 354, 411.
  6. DeFazio, R.A. et al. (2000) Neuroscience, 99, 297.
  7. Belluardo, N. et al. (2001) Mol. Cell Neurosci. 18, 56.
  8. Spalding, K.L. et al. (2002) Mol. Cell Neurosci. 19, 485.
  9. Lobner, D. and Ali, C. (2002) Brain Res. 954, 42.
  10. Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.
Scientific background

The neurotrophins ("neuro" meaning nerve and "trophe" meaning nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.1 Neurotrophin-4 (NT-4) is expressed in neurons of the superior cervical, stellate and celiac ganglion,2 T-cells3 and is synthesized by keratinocytes.4 The structural hallmark of all neurotrophins is the characteristic arrangement of the disulfide bridges, known as the cysteine knot, which has been found in other growth factors such as PDGF.5 

The rat and human forms of NT-4 are 96% homologous. NT-4 has been shown to promote dendritic outgrowth and calcium currents in cultured mesencephalic dopamine neurons,6 to promote growth and remodeling of adult motor neuron innervation,7 to be anterograde survival factors for postsynaptic cells8 and to protect against apoptotic neuronal death.9 

The biological effects of NT-4 are mediated by two receptors: TrkB, which is specific for NT-4 and BDNF, and p75NTR, which binds all the neurotrophins.10

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/06/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Neurotrophin 4. Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004) can be used in western blot and immunohistochemistry applications. It has been designed to recognize NT-4 from rat, mouse and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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