Anti-Neurotrophin 4 (NT-4) Antibody

NTF4, Neutrophic factor 4, Neurotrophin 5, NT-5, NTF5, Neutrophic factor 5, NT 4/5
    Cat #: ANT-004
    Alternative Name NTF4, Neutrophic factor 4, Neurotrophin 5, NT-5, NTF5, Neutrophic factor 5, NT 4/5
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide LGEVPAAGGSPL(C), corresponding to amino acid residues 43-52 of mature human NT-4 (residues 123-132 of the precursor NT-4) (Accession P34130).
    • Anti-Neurotrophin 4 (NT-4) Antibody
    Accession (Uniprot) Number P34130
    Gene ID 16485
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - identical.
    RRID AB_2040017.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.3 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 0.5 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Anti-Neurotrophin 4 (NT-4) Antibody
      Western blot analysis of Recombinant human Neurotrophin-4 (NT-4) protein (#N-270), (10 ng):
      1. Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004), (1:200).
      2. Anti-Neurotrophin 4 (NT-4) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-Neurotrophin 4 (NT-4) Antibody
      Expression of Neurotrophin 4 in mouse spinal cord
      Immunohistochemical staining of mouse spinal cord using Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004). The spinal ventral horn (VH) was very lightly stained. However, in the perimeter of the white matter (WM) there were NT-4 immunoreactive cells with a small soma (triangles) and some had a stained process (arrows).
    References
    1. Roux, P. et al. (2002) Prog. Neurobiol. 67, 203.
    2. Roosen, A. et al. (2001) J. Neurosci. 21, 3073.
    3. Moalem, G. et al. (2002) J. Autoimmun. 15, 331.
    4. Marconi, A. et al. (2003) J. Invest. Dermatol. 121, 1515.
    5. McDonald, N.Q. et al. (1991) Nature 354, 411.
    6. DeFazio, R.A. et al. (2000) Neuroscience, 99, 297.
    7. Belluardo, N. et al. (2001) Mol. Cell Neurosci. 18, 56.
    8. Spalding, K.L. et al. (2002) Mol. Cell Neurosci. 19, 485.
    9. Lobner, D. and Ali, C. (2002) Brain Res. 954, 42.
    10. Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.
    Scientific background

    The neurotrophins ("neuro" meaning nerve and "trophe" meaning nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.1 Neurotrophin-4 (NT-4) is expressed in neurons of the superior cervical, stellate and celiac ganglion,2 T-cells3 and is synthesized by keratinocytes.4 The structural hallmark of all neurotrophins is the characteristic arrangement of the disulfide bridges, known as the cysteine knot, which has been found in other growth factors such as PDGF.5 

    The rat and human forms of NT-4 are 96% homologous. NT-4 has been shown to promote dendritic outgrowth and calcium currents in cultured mesencephalic dopamine neurons,6 to promote growth and remodeling of adult motor neuron innervation,7 to be anterograde survival factors for postsynaptic cells8 and to protect against apoptotic neuronal death.9 

    The biological effects of NT-4 are mediated by two receptors: TrkB, which is specific for NT-4 and BDNF, and p75NTR, which binds all the neurotrophins.10

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Neurotrophin 4. Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004) can be used in western blot and immunohistochemistry applications. It has been designed to recognize NT-4 from rat, mouse and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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