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- Peptide (C)VEKQSTDSGAYSIGH, corresponding to amino acid residues 383-397 of rat P2X3 receptor (Accession P49654). Intracellular, C-terminus.
- Expression of P2RX3 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-P2X3 Receptor-ATTO-594 Antibody (#APR-016-AR). A. P2X3 staining (red) is detected in DRG neurons. B. Nuclear staining using DAPI as the counterstain (Blue). C. Merge images of A and B.
- 1. Florenzano, F. et al. (2002) Neuroscience 115, 425.
- 2. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed.1.
- 3. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107.
- 4. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
- 5. Kage, K. et al. (2002) Exp. Brain Res. 147, 511.
- 6. Prasad, M. et al. (2001) J. Physiol. 573, 667.
- 7. Aoki, Y. et al. (2003) Brain Res. 989, 214.
- 8. Burnstock, G. (2000) Br. J. Anaesth. 84, 476.
The P2X3 receptor belongs to the ligand-gated ion channel P2X receptor family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP.1,2,3
All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals.6,7 Involvement of ATP in the mechanism of chronic pain has been also suggested.7,8 P2X3 receptor is now becoming a possible target for the development of pain therapeutics.
Species reactivity key:
Anti-P2X3 Receptor Antibody (#APR-016) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize P2X3 receptor from rat, mouse, and human samples.
Anti-P2X3 Receptor-ATTO-594 Antibody (#APR-016-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-P2X3 Receptor-ATTO-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.
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