Overview
- Peptide (C)KGFGRYANRVMDVSD, corresponding to amino acid residues 65-79 of rat P2X3 receptor (Accession P49654). Extracellular.
Cell surface detection of P2X3 Receptor by direct flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-P2X3 Receptor (extracellular)-FITC Antibody (#APR-026-F), (5µg).
Cell surface detection of P2X3 Receptor by direct flow cytometry in live intact mouse P815 mastocytoma cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-P2X3 Receptor (extracellular)-FITC Antibody (#APR-026-F), (5µg).
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The P2X3 receptor belongs to the ligand-gated ion channel P2X receptor family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP.1,2,3
All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals.6,7 Involvement of ATP in the mechanism of chronic pain has been also suggested.7,8 P2X3 receptor is now becoming a possible target for the development of pain therapeutics.
Anti-P2X3 Receptor (extracellular) Antibody (#APR-026) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and live cell flow cytometry applications. It has been designed to recognize P2X3 receptor from rat, mouse and human samples.
Anti-P2X3 Receptor (extracellular)-FITC Antibody (#APR-026-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize P2X3 receptor from rat, mouse and human samples.
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