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Anti-Human P2Y11 Receptor Antibody

P2Y purinoceptor 11, P2RY11

Cat #: APR-015
Alternative Name P2Y purinoceptor 11, P2RY11
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide (C)NATAAPKPSEPQSRELS, corresponding to amino acid residues 357-373 of human P2RY11 (Accession Q96G91). Intracellular, C-terminus.
Accession (Uniprot) Number Q96G91
Gene ID 5032
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human only. Not recommended for use with rat and mouse samples.
RRID AB_2040072.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.9 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ip, wb
May also work in: ih*
Western blot
  • Western blot analysis of human platelet lysates:
    Western blot analysis of human platelet lysates:
    1. Anti-Human P2Y11 Receptor Antibody (#APR-015), (1:200).
    2. Anti-Human P2Y11 Receptor Antibody, preincubated with Human P2Y11 Receptor Blocking Peptide (#BLP-PR015).
Immunoprecipitation
  • Mouse bone marrow lysate (Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.).
Immunocytochemistry
  • Expression of P2Y11 receptor in human P2Y11-MDCK transfected cells
    Expression of P2Y11 receptor in human P2Y11-MDCK transfected cells
    Immunocytochemical staining of paraformaldehyde-fixed and permeabilized human P2Y11-MDCK transfected cells. A. Staining using Anti-Human P2Y11 Receptor Antibody (#APR-015), (1:200), followed by Alexa-488-conjugated goat-anti-rabbit secondary antibody. B. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B.
Indirect flow cytometry
  • Rat osteoclasts (6 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
  • The blocking peptide is not suitable for this application.
References
  1. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
  2. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
  3. Abbracchio, M.P. et al. (2003) Trends. Pharmacol. Sci. 24, 52.
  4. Communi, D. et al. (2000) FEBS Letters 475, 39.
  5. White, P.J. et al. (2003) Mol. Pharmacol. 63, 1356.
  6. Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841.
  7. IUPHAR.
Scientific background

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP). Eight functional mammalian P2Y receptors have been described: P2Y1P2Y2P2Y4P2Y6, P2Y11, P2Y12P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3 

The human P2Y11 receptor is unique among the P2Y receptors since it is dually coupled to phospholipase C and adenylyl cyclase.4-5 P2Y11 receptor is highly expressed in the spleen and to a much lower extent in platelets.

Expression of P2Y11 receptor was also shown in endothelial cells.6 In granulocytes HL-60), P2Y11 receptor is thought to have a role in granulocytic differentiation.4,7

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-Human P2Y11 Receptor Antibody (#APR-015) is a highly specific antibody directed against an epitope of the human P2RY11. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, and immunocytochemistry applications. It has been designed to recognize P2RY11 from human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Mouse bone marrow lysate.
    Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
  2. Rat cardiac fibroblast lysate (1:200).
    Certal, M. et al. (2015) Cell Calcium 58, 518.
  3. Human PBMC lysate (1:600).
    Chadet, S. et al. (2015) J. Immunol. 195, 651.
  4. Human derived keratinocytes (HaCaT cells), (1:200).
    Ishimaru, M. et al. (2013) Eur. J. Pharmacol. 703, 67.
Immunoprecipitation citations
  1. Mouse bone marrow lysate.
    Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
Immunocytochemistry citations
  1. Rat cardiac fibroblasts (1:50).
    Certal, M. et al. (2015) Cell Calcium 58, 518.
Indirect flow cytometry citations
  1. Mouse bone marrow cells.
    Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
  2. Rat osteoclasts (6 μg/ml).
    Alvarenga, E.C. et al. (2010) Bone 46, 355.
More product citations
  1. Zerpa, H. et al. (2013) Eur. J. Pharmacol. 702, 242.
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