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Anti-P2Y12 Receptor (extracellular) Antibody

P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3
Cat #: APR-020
Alternative Name P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
May also work in: m*
Immunogen
  • Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12 (Accession Q9H244). 3rd extracellular loop.
Accession (Uniprot) Number Q9H244
Gene ID 64805
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse - 12/13 amino acid residues identical.
RRID AB_11121048.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, lci, wb
May also work in: ih*, ip*
Western blot
  • Western blot analysis of rat brain membrane (lanes 1 and 3) and MEG-01 (lanes 2 and 4) cell line lysate:
    Western blot analysis of rat brain membrane (lanes 1 and 3) and MEG-01 (lanes 2 and 4) cell line lysate:
    1-2. Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020), (1:200).
    3-4. Anti-P2Y12 Receptor (extracellular) Antibody, preincubated with P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020).
Indirect flow cytometry
  • Cell surface detection of P2RY12 in live intact human MEG-O1 cells:
    Cell surface detection of P2RY12 in live intact human MEG-O1 cells:
    ___ Unstained cells.
    ___ Cells + Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020), (5-10 µg/1x106 cells).
  • The blocking peptide in not suitable for this application.
Live cell imaging / Immunocytochemistry
  • Expression of P2RY12 in rat RBL cells
    Expression of P2RY12 in rat RBL cells
    Cell surface detection of P2RY12 in intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020), (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.
References
  1. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
  2. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
  3. Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
  4. Nicholas, R.A. (2001) Mol. Pharmacol. 60, 416.
Scientific background

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1P2Y2P2Y4P2Y6P2Y11P2Y12P2Y13, and the UDP-glucose receptor, now renamed P2Y141-3

The P2Y12 receptor is co-expressed with the P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.

The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting4. The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:


Expression of P2Y12 Receptor in mouse BV-2 microglia cells.Cell surface detection of P2Y12 Receptor in live intact mouse BV-2 microglia cells using Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020). P2Y12 receptor immunoreactivity (green) is detected at the cell surface of cells.Adapted from Jiang, P. et al. (2017) PLoS ONE 12, e0183114. with permission of PLos. 

Last update: 16/08/2020

Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) is a highly specific antibody directed against an extracellular epitope of the human P2RY12. The antibody can be used in western blot, live cell imaging and indirect flow cytometry applications. It has been designed to recognize P2RY12 from human, rat and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Live cell imaging citations
  1. Mouse BV-2 microglia cells.
    Jiang, P. et al. (2017) PLoS ONE 12, e0183114.
Immunofluorescence citations
  1. Mouse BV-2 microglia cells.
    Jiang, P. et al. (2017) PLoS ONE 12, e0183114.
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