Anti-P2Y4 Receptor Antibody

P2Y purinoceptor 4, P2RY4, Uridine nucleotide receptor
    Cat #: APR-006
    Alternative Name P2Y purinoceptor 4, P2RY4, Uridine nucleotide receptor
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)HEES ISRWA DTHQD, corresponding to residues 337-350 of rat P2RY4 (Accession O35811). Intracellular, C-terminus.
    • Anti-P2Y4 Receptor Antibody
    Accession (Uniprot) Number O35811
    Gene ID 63843
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 13/14 amino acid residues identical.
    RRID AB_2040080.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.9 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, ih, ip, wb
    Western blot
    • Anti-P2Y4 Receptor Antibody
      Western blot analysis of rat brain membranes:
      1. Anti-P2Y4 Receptor Antibody (#APR-006), (1:300).
      2. Anti-P2Y4 Receptor Antibody, preincubated with the control peptide antigen.
    Immunoprecipitation
    • HEK-293 transfected cells (1 μg) (Cavaliere, F. et al. (2004) Exp. Cell Res300, 149.).
    Immunohistochemistry
    • Anti-P2Y4 Receptor Antibody
      Expression of P2RY4 in rat cerebellum
      Immunohistochemical staining of rat cerebellum using Anti-P2Y4 Receptor Antibody (#APR-006). A. P2RY4 (red) appears in astrocytic processes (arrows). B. In contrast, P2RY4 does not appear in Purkinje neuronal dendrites stained for calbindin D28k (green) in the same brain section. This demonstrates the restriction of P2RY4 to astrocytic fibers.
    • Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
    Immunocytochemistry
    • Mouse macrophage J774 (Coutinho Silva, R. et al. (2005) Biochem. Pharmacol69, 641.).
    Indirect flow cytometry
    • Rat osteoclasts (3 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
    • The control antigen is not suitable for this application.
    References
    1. Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841.
    2. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
    3. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
    4. Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
    5. Von Kugelgen,I. and Wetter, A. (2000) Naunyn Schmiedebergs Arch Pharmacol. 362, 310.
    6. Cavaliere, F. et al. (2005) Neurobiol. Dis. 18, 100.
    Scientific background

    The P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation.1

    The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4

    The P2Y4-receptor is highly expressed in the placenta and with lower levels in the lung and vascular smooth muscle.5

    The human P2Y4 receptor is highly selective to UTP while rat P2Y4 receptor is equally activated by both UTP and ATP and also by other triphosphate nucleotides such as Ap4A.5

    Recently it has been shown that P2Y4 receptor is involved in differentiation and cell death of human neuroblastoma SH-SY5Y cells.6

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-P2Y4 Receptor Antibody (#APR-006) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2RY4 in rat, mouse, and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Mouse bone marrow lysate.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    2. Mouse bone lysate and osteoblast MOB-C cell lysate.
      Seref-Ferlengez, Z. et al. (2016) PLoS ONE 11, e0155107.
    3. Rat cardiac fibroblasts (1:300).
      Certal, M. et al. (2015) Cell Calcium 58, 518.
    Immunoprecipitation citations
    1. Mouse bone marrow lysate.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    2. HEK-293 transfected cells (1 μg).
      Cavaliere, F. et al. (2004) Exper. Cell Res. 300, 149.
    Immunohistochemistry citations
    1. Human eye tissue.
      Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.
    Immunocytochemistry citations
    1. Rat cardiac fibrobalsts (1:75).
      Certal, M. et al. (2015) Cell Calcium 58, 518.
    2. Mouse macrophage J774.
      Coutinho Silva, R. et al. (2005) Biochem. Pharmacol. 69, 641.
    Indirect flow cytometry citations
    1. Mouse bone marrow cells.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    2. Rat osteoclasts (3 μg/ml).
      Alvarenga, E.C. et al. (2010) Bone 46, 355.
    More product citations
    1. Traves, P.G. et al. (2013) J. Immunol. 190, 4226.
    2. Zerpa, H. et al. (2013) Eur. J. Pharmacol. 702, 242.
    3. Sage, C.L. and Marcus D. C. (2002) Membr. Biol. 185, 103.
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