Anti-Pan NaV Antibody

Pan sodium channel, SP19
    Cat #: ASC-003
    Alternative Name Pan sodium channel, SP19
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat NaV1.1 (Accession P04774). Intracellular loop between domains III and IV.
        Anti-Pan NaV Antibody
    Accession (Uniprot) Number P04774
    Gene ID 81574
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology The epitope is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels.
    RRID AB_2040204.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: fc*
      • Anti-Pan NaV Antibody
        Western blot analysis of rat brain membranes:
        1. Anti-Pan NaV Antibody (#ASC-003), (1:200).
        2. Anti-Pan NaAntibody, preincubated with the negative control antigen.
        Human atrial myofibroblasts (1:1000). (Chatelier, A. et al. (2012) J. Physiol. 590, 4307.).
      • Rat brain (Anis, Y. et al. (1999) J. Biol. Chem. 274, 7431.).
      • Anti-Pan NaV Antibody
        Expression of NaV channels in rat cerebellum
        Immunohistochemical staining of rat cerebellum using Anti-Pan NaV Antibody (#ASC-003). A. NaV channels (red) appear in Purkinje cell bodies (vertical arrows) and in cells of the molecular layer (Mol), (horizontal arrows). B. Staining of Parvalbumin (green) in the same brain section. C. Confocal merge of Nachannels and Parvalbumin demonstrates the location of NaV channels to the cell body of Purkinje neurons.
      • PC-3 human prostate epithelial tumor cell lines and Mat-LyLu rat epithelial tumor cell lines (Nakajima, T. et al. (2009) Br. J. Pharmacol. 156, 420.).
    References
      • Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5  The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3

        Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer an antibody directed against an intracellular epitope of the rat NaV1.1 channel that is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels. Anti-Pan NaV Antibody (#ASC-003) can be used in western blot, immunocytochemistry, and immunohistochemistry applications.

    For research purposes only, not for human use
    Citations
      • Rat brain neurolemma lysate.
        Murenzi, E. et al. (2017) Neurotoxicology 60, 260.
      • Mouse neuron-derived CAD transfected cells.
        Dustrude, E.T. et al. (2016) Proc. Natl. Acad. Sci. U.S.A. 113, E8443.
      • Mouse brain lysate (1:200).
        Ban, Y. et al. (2015) J. Neurophysiol. 114, 520.
      • Mouse neuronal catecholamine A differentiated cells (CAD).
        Dustrude, E.T. et al. (2013) J. Biol. Chem. 288, 24316.
      • Human atrial myofibroblasts (1:1000).
        Chatelier, A. et al. (2012) J. Physiol. 590, 4307.
      • Rat brain.
        Anis, Y. et al. (1999) J. Biol. Chem. 274, 7431.
      • Dog heart.
        Yue, L. et al. (1999) Circ Res. 84, 776.
      • Rat brain.
        Anis, Y. et al. (1999) J. Biol. Chem. 274, 7431.
      • Mouse cochleae sections.
        Smith, K.E. et al. (2015) J. Neurosci. 35, 11221.
      • Guinea pig intestine.
        Hanani, M. et al. (2000) Am J. Physiol. 278, G644.
      • Rat hippocampus cell culture.
        Sobieski, C. et al. (2015) J. Neurosci. 35, 11105.
      • Human PC3 prostate epithelial tumor cell line.
        Nakajima, T. et al. (2009) Br. J. Pharmacol. 156, 420.
      • Rat Mat-LyLu epithelial tumor cell line.
        Nakajima, T. et al. (2009) Br. J. Pharmacol. 156, 420.
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