Anti-SAP-97/DLG1 Antibody

Synapse-associated protein 97, Discs large homolog 1
    Cat #: APZ-010
    Alternative Name Synapse-associated protein 97, Discs large homolog 1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)EYRSKLSQTEDRQLR, corresponding to amino acid residues 17-31 of human SAP-97 (Accession Q12959). N-terminus, L27 domain.
        Anti-SAP-97/DLG1 Antibody
    Accession (Uniprot) Number Q12959
    Gene ID 1739
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - identical.
    RRID AB_2756771.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
      • Anti-SAP-97/DLG1 Antibody
        Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6):
        1-3. Anti-SAP-97/DLG1 Antibody (#APZ-010), (1:200).
        4-6. Anti-SAP-97/DLG1 Antibody, preincubated with the negative control antigen.
      • SAP-97 belongs to the MAGUK (membrane associated guanylate kinase) homologs along with PSD-95PSD-93 and SAP102.

        Like other members, SAP-97 has three PDZ domains, one SH3 domain and an inactive GUK domain. Alternative splicing of SAP-97 gives to a number of different isoforms thereby complicating the understanding of its roles1.

        In the post-synaptic cell, SAP-97 is the only MAGUK to directly interact with the GluA1 AMPA receptor2. SAP-97 also directly interacts with NMDA receptors through the PDZ domain3,4.

        SAP-97 was shown to interact with KV1.45 and with KV4.2 where it directs the channel to spines in hippocampal neurons6. It can also interact with the channel in the heart7.

        Interactions with Kir channels have also been observed8,9.

        Thus, SAP-97 serves as a regulator of neuronal excitability and synaptic function. Roles for the protein in schizophrenia, Alzheimer’s and Parkinson’s disease and epilepsy have been found1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human SAP-97. Anti-SAP-97/DLG1 Antibody (#APZ-010) can be used in western blot analysis. It has been designed to recognize SAP-97 from rat, mouse and human samples.

    For research purposes only, not for human use