Anti-Somatostatin Receptor Type 2 Antibody

SSTR2a, SS2R, Smstr2, SRIF1A
    Cat #: ASR-002
    Alternative Name SSTR2a, SS2R, Smstr2, SRIF1A
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)ERSDSKQDKSRLNETTETQRT corresponding to residues 339-359 of rat SSTR2 (Accession P30680). Intracellular, C-terminus.
    • Anti-Somatostatin Receptor Type 2 Antibody
    Accession (Uniprot) Number P30680
    Gene ID 54305
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    RRID AB_2341073.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Anti-Somatostatin Receptor Type 2 Antibody
      Western blot analysis of rat brain membranes: 
      1. Anti-Somatostatin Receptor Type 2 Antibody (#ASR-002), (1:400).
      2. Anti-Somatostatin Receptor Type 2 Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Canine meningioma sections (Foiani, G. et al. (2019) J. Comp. Pathol. 166, 59.).

    References
    1. Hofland, L.J. and Lamberts, S.W. (2001) Ann. Oncol. 12, S31.
    2. Fombonne, J. et al. (2003) Reprod. Biol. Endocrinol. 1, 19.
    3. Slooter, G.D. et al. (2001) Br. J. Surg. 88, 31.
    4. Schulz, S. et al. (2002) Gyn. Oncol. 84, 235.
    Scientific background

    Somatostatin is a small cyclic peptide that is widely expressed throughout the central nervous system and peripheral tissues1. In peripheral tissues, somatostatin exerts inhibitory effects on secretion processes, whereas in the brain, it acts as a neurotransmitter in both a stimulatory and an inhibitory manner1,2.

    Somatostatin mediates its action via six high affinity G-protein coupled receptors (SSTR1, SSTR2a, SSTR2b, SSTR3SSTR4, and SSTR5), which are encoded by five genes1,2. Expression of the different receptors is developmentally regulated in a time- and tissue-specific manner2.

    Somatostatin receptors have been found on a variety of neuroendocrine tumors, such as paragangliomas, carcinoids, and breast tumors3. Synthetic peptide derivatives of somatostatin have been successfully used in the treatment of neuroendocrine malignancies and in vivo imaging of tumors that are positive for somatostatin receptors4.

    In general, SSTR2 is the most common SSTR subtype found in human tumors, followed by SSTR1, with SSTR3 and SSTR4 being less common.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Somatostatin Receptor Type 2 Antibody (#ASR-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SSTR2 from human, mouse, and rat samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Canine meningioma culture lysate.
      Foiani, G. et al. (2019) J. Comp. Pathol. 166, 59.
    Immunohistochemistry citations
    1. Canine meningioma sections.
      Foiani, G. et al. (2019) J. Comp. Pathol. 166, 59.
    Shipping and Ordering information