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Anti-S1PR1 (EDG1) (extracellular) Antibody

Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CD363, CHEDG1

Cat #: ASR-011
Alternative Name Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CD363, CHEDG1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide SDYVNYDIIVRHYN(C), corresponding to amino acid residues 17-30 of human S1PR1 (Accession P21453). Extracellular, N-terminus.
Accession (Uniprot) Number P21453
Gene ID 1901
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat - 13/14 amino acid residues identical.
RRID AB_2039836.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, lci, wb
May also work in: ip*
Western blot
  • Western blot analysis of (RAEC) rat aortic endothelial  cell line lysates (lanes 1 and 3) and A-10 Rat thoracic aorta smooth muscle (lane 2 and 4) cell lysates:
    Western blot analysis of (RAEC) rat aortic endothelial  cell line lysates (lanes 1 and 3) and A-10 Rat thoracic aorta smooth muscle (lane 2 and 4) cell lysates:
    1,2. Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011), (1:200).
    3,4. Anti-S1PR1 (EDG1) (extracellular) Antibody, preincubated with S1PR1/EDG1 (extracellular) Blocking Peptide (#BLP-SR011).
Immunohistochemistry
  • Expression of Sphingosine 1-phosphate receptor 1 in rat lung
    Expression of Sphingosine 1-phosphate receptor 1 in rat lung
    Immunohistochemical staining of paraffin embedded rat lung sections using Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011), (1:100). Staining is present in vascular smooth muscle (black arrows) but not in the muscular layer of bronchi (red arrows). Hematoxilin is used as the counterstain.
Indirect flow cytometry
  • Cell surface detection of Sphingosine 1-phosphate receptor 1 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of Sphingosine 1-phosphate receptor 1 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-APC.
    ___ Cells + Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011), (5μg) + goat-anti-rabbit-APC.
Live cell imaging / Immunocytochemistry
  • Expression of Sphingosine 1-phosphate receptor 1 in mouse 3T3 cells
    Expression of Sphingosine 1-phosphate receptor 1 in mouse 3T3 cells
    Cell surface detection of Sphingosine 1-phosphate receptor 1 in mouse live 3T3 cells. A. Extracellular staining of cells using Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011), (1:50), (red). B. DAPI is used for nuclear staining (blue). Merged image of A and B.
References
  1. Pyne, S. and Pyne, N.J. (2000) Biochem. J. 349, 385.
  2. Hla, T. et al. (2001) Science 294, 1875.
  3. Takuwa, Y. et al. (2002) J. Biochem. 131, 767.
  4. Rosen, H. et al. (2009) Annu. Rev. Biochem. 78, 743.
  5. Rosen, H and Liao, J. (2003) Curr. Opin. Chem. Biol. 7, 461.
Scientific background

Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2. Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.

Sphingosine 1-phosphate receptor 1 (S1PR1) is ubiquitously expressed. S1PR1 mRNA is detected in the brain, heart, spleen, liver, lung, thymus, kidney, skeletal muscle and lymphoid tissues4. Knockout of the gene encoding S1PR1 is lethal and therefore makes in vivo studies difficult5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 02/08/2022

Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize S1PR1 from human, rat, and mouse samples.

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For research purposes only, not for human use

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Scientific Background

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