Anti-STOML2/SLP-2 Antibody

Paraprotein target 7, EPB72-like protein 2, Stomatin-like protein 2
    Cat #: AIP-002
    Alternative Name Paraprotein target 7, EPB72-like protein 2, Stomatin-like protein 2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide (C)EGTRESAINVAEGKK, corresponding to amino acid residues 208-222 of rat Stomatin-like protein 2 (Accession Q4FZT0). Intracellular.
    • Anti-STOML2/SLP-2 Antibody
    Accession (Uniprot) Number Q4FZT0
    Gene ID 298203
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ih*, ip*
    Western blot
    • Anti-STOML2/SLP-2 Antibody
      Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and rat skeletal muscle lysate (lanes 3 and 6):
      1-3. Anti-STOML2/SLP-2 Antibody (#AIP-002), (1:400).
      4-6. Anti-STOML2/SLP-2 Antibody, preincubated with the negative control antigen.
    • Anti-STOML2/SLP-2 Antibody
      Western blot analysis of human brain neuroblastoma SH-SY5Y (lanes 1 and 5), human prostate carcinoma DU 145 (lanes 2 and 6), human pancreatic carcinoma PANC-1 (lanes 3 and 7) and human acute lymphoblastic leukemia MOLT-4 (lanes 4 and 8):
      1-4. Anti-STOML2/SLP-2 Antibody (#AIP-002), (1:800).
      5-8. Anti-STOML2/SLP-2 Antibody, preincubated with the negative control antigen.
    1. Zhu, W. et al. (2018) Med. Sci. Monit. 24, 7625.
    2. Sun, F. et al. (2015) BMC Cancer 15, 746.
    3. Wang, Y. and Morrow, J.S. (2000) J. Biol. Chem. 275, 8062.
    Scientific background

    Stomatin-like protein 2 (STOML2, SLP-2) is a key protein on the mitochondrial inner membrane and a member of the stomatin superfamily. STOML2 is widely expressed in human tissues such as the brain, lung, and kidneys and interacts with prohibitin-1 and 2. It plays an important role in numerous physiological processes including organizing sphingolipid and cholesterol-rich lipid rafts, regulating ion channel conductance, and linking other integral membrane proteins to the peripheral cytoskeleton1,2.

    STOML2 shares a consensus stomatin signature sequence that defines the stomatin gene family. However, it lacks a NH2-terminal hydrophobic domain shared by other members3.

    Studies show that STOML2 expression is upregulated in various human cancer tissues including gastric cancer, endometrial adenocarcinoma, and breast cancer. The protein may play an important role in the incidence and development of tumor and it is suggested that SLP-2 is a potential oncogene. Knockdown of STOML2 protein significantly restrains the viability, migration, and invasion of hepatoma cell line1,2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-STOML2/SLP-2 Antibody (#AIP-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Stomatin-like protein 2 from human, rat, and mouse samples.

    For research purposes only, not for human use



    Scientific Background


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