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Anti-TRPC4 Antibody

Transient receptor potential channel 4, TRP4, hTrp4, Capacitative calcium entry channel Trp4, CCE1

Cat #: ACC-018
Alternative Name Transient receptor potential channel 4, TRP4, hTrp4, Capacitative calcium entry channel Trp4, CCE1
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)KEKHAHEEDSSIDYDL, corresponding to amino acid residues 943-958 of mouse TRPC4 (Accession Q9QUQ5). Intracellular, C-terminus.
    Accession (Uniprot) Number Q9QUQ5
    Gene ID 22066
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human - 15/16 amino acid residues identical; bovine -  11/15 amino acid residues identical.
    RRID AB_2040239.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.4 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: ifc*
    Western blot
    • Western blot analysis of rat brain membranes:
      Western blot analysis of rat brain membranes:
      1. Anti-TRPC4 Antibody (#ACC-018), (1:200).
      2. Anti-TRPC4 Antibody, preincubated with TRPC4 Blocking Peptide (#BLP-CC018).
    • Western blot analysis of human PC3 Caucasian prostate adenocarcinoma (lanes 1 and 3) and human LNCaP prostate carcinoma (lanes 2 and 4) cell lysates:
      Western blot analysis of human PC3 Caucasian prostate adenocarcinoma (lanes 1 and 3) and human LNCaP prostate carcinoma (lanes 2 and 4) cell lysates:
      1,3. Anti-TRPC4 Antibody (#ACC-018), (1:200).
      2,4. Anti-TRPC4 Antibody, preincubated with TRPC4 Blocking Peptide (#BLP-CC018).
    Immunoprecipitation
    • Immunoprecipitation of rat brain lysate:
      Immunoprecipitation of rat brain lysate:
      1. Rat brain lysate.
      2. Lysate immunoprecipitated with Anti-TRPC4 Antibody (#ACC-018), (6 µg).
      3. Lysate immunoprecipitated with pre-immune rabbit serum.
      The upper arrow indicates the TRPC4 channel while the lower arrow indicates the IgG heavy chain.
      Western blot analysis was performed with Anti-TRPC4 Antibody.
    Immunohistochemistry
    • Expression of TRPC4 in mouse cerebellum
      Expression of TRPC4 in mouse cerebellum
      Immunohistochemical staining of mouse cerebellum frozen sections with Anti-TRPC4 Antibody (#ACC-018). A. TRPC4 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) layer. B. Staining with mouse anti-parvalbumin (PV) in the same brain section. C. Confocal merge of TRPC4 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.
    Immunocytochemistry
    • Rat type I astrocytes (1:25) (Grimaldi, M. et al. (2003) J. Neurosci. 23, 4737.).  

      Human U373 MG cells (1:100) (Barajas, M. et al. (2008) J. Neurosci. Res. 86, 3456.).
    References
    1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362.
    2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
    3. Clapham, D.E. (2003) Nature 426, 517.
    4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707.
    5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690.
    6. Liu, X. et al. (2003) J. Biol.Chem. 278, 11337.
    Scientific background

    The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4 

    The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities:
    1. TRPC1
    2. TRPC4 and TRPC5
    3. TRPC3TRPC6TRPC7
    4. TRPC2.2,5 
    They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.

    TRPC4 can form heterotetramers with TRPC1. TRPC4, TRPC1 and TRPC5 can be activated either by calcium store depletion or by GPCR stimulation pathways and are also assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells6

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-TRPC4 Antibody
    Knockout validation of Anti-TRPC4 Antibody in mouse smooth muscle cells.Immunocytochemical staining of mouse smooth muscle cells with Anti-TRPC4 Antibody (#ACC-018). TRPC4 staining is prominent in wild type cells (left panels). TRPC4 is not detected in TRPC4-/- cells (right panels) demonstrating the specificity of the antibody.Adapted from Griffin, C.S. et al. (2018) Sci. Rep. 8, 9264. with permission of SPRINGER NATURE.

    Last update: 12/08/2021

    Anti-TRPC4 Antibody (#ACC-018) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRPC4 from human, mouse, and rat samples.

    For research purposes only, not for human use

    Applications

    Citations

    Citations
    Published figures using this product
    • Expression of TRPC4 in human MEC-1 cells.
      Expression of TRPC4 in human MEC-1 cells.
      Immunocytochemical staining of human microvascular endothelial cells (HMEC-1) using Anti-TRPC4 Antibody (#ACC-018).
      Adapted from Graziani, A. et al. (2010) J. Biol. Chem. 285, 4213. with permission of The American Society for Biochemistry and Molecular Biology.
    KO validation citations
    1. Immunocytochemical staining of mouse smooth muscle cells. Also tested in TRPC4-/- cells.
      Griffin, C.S. et al. (2018) Sci. Rep. 8, 9264.
    2. Western blot analysis of rat pulmonary artery lysates. Also tested in siRNA treated cells.
      Jiang, H.N. et al. (2016) Biomed. Pharmacol. 82, 20.
    Western blot citations
    1. Rat pulmonary artery lysates. Also tested in siRNA treated cells.
      Jiang, H.N. et al. (2016) Biomed. Pharmacol. 82, 20.
    2. Rat neonatal ventricular cardiomyocytes (1:200).
      Sabourin, J. et al. (2016) J. Biol. Chem. 291, 13394.
    3. Rat INS-1 cell lysate.
      Park, S.H. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 12673.
    4. Mouse brain lysate.
      Feng, S. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 11011.
    5. Human primary myotubes.
      Harisseh, R. et al. (2013) Am. J. Physiol. 304, C881.
    6. Rat distal pulmonary smooth muscle cell lysate (PASMCs).
      Zhang, Y. et al. (2013) Am. J. Physiol. 304, C833.
    Immunoprecipitation citations
    1. Rat neonatal ventricular cardiomyocytes.
      Sabourin, J. et al. (2016) J. Biol. Chem. 291, 13394.
    Immunocytochemistry citations
    1. Mouse smooth muscle cells. Also tested in TRPC4-/- cells.
      Griffin, C.S. et al. (2018) Sci. Rep. 8, 9264.
    2. Rat primary cultured fetal and neonatal ventricular myocytes (1:100).
      Jiang, Y. et al. (2014) Cell Tissue Res. 355, 201.
    3. Human primary myotubes.
      Harisseh, R. et al. (2013) Am. J. Physiol. 304, C881.
    4. Human U373 MG cells (1:100).
      Barajas, M. et al. (2008) J. Neurosci. Res. 86, 3456.
    5. Rat type I astrocytes (1:25).
      Grimaldi, M. et al. (2003) J. Neurosci. 23, 4737.
    More product citations
    1. Kadeba, P.I. et al. (2013) Cell Calcium 53, 275.
    2. Graziani, A. et al. (2010) J. Biol. Chem. 285, 4213.
    3. Facemire, C.S. et al . (2004) Am. J. Physiol. 286, F546.
    4. Castellano, L.E. et al. (2003) FEBS Lett. 541, 69.
    5. Vandebrouck, C. et al. (2002) J. Cell. Biol. 158, 1089.
    6. Wu, X. et al. (2002) J. Biol. Chem. 277, 13597.

    Specifications

    Scientific Background

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