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Anti-TRPM4 Antibody

Melastatin-4, LTrpC4, Calcium-activated non-selective cation channel 1

Cat #: ACC-044
Alternative Name Melastatin-4, LTrpC4, Calcium-activated non-selective cation channel 1
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide EKEQSWIPKIFKK(C), corresponding to amino acid residues 5-17 of human TRPM4 (Accession Q8TD43). Intracellular, N-terminus.
    Accession (Uniprot) Number Q8TD43
    Gene ID 54795
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - 12/13 amino acid residues identical.
    RRID AB_2040250.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
    Western blot
    • Western blot analysis of rat lung (lanes 1 and 5), rat heart membrane (lanes 2 and 6), mouse brain membrane (lanes 3 and 7) and ms1 mouse pancreas cells lysate (lanes 4 and 8):
      Western blot analysis of rat lung (lanes 1 and 5), rat heart membrane (lanes 2 and 6), mouse brain membrane (lanes 3 and 7) and ms1 mouse pancreas cells lysate (lanes 4 and 8):
      1-4. Anti-TRPM4 Antibody (#ACC-044), (1:200).
      5-8. Anti-TRPM4 Antibody, preincubated with TRPM4 Blocking Peptide (#BLP-CC044).
    Immunohistochemistry
    • Expression of TRPM4 in rat lung
      Expression of TRPM4 in rat lung
      Immunohistochemical staining of rat lung paraffin embedded sections using Anti-TRPM4 Antibody (#ACC-044), (1:100). TRPM4 is expressed in the respiratory epithelium of the bronchioli (arrows). Hematoxilin is used as the counterstain.
    References
    1. Fleig, A. et al. (2004) Trends. Pharmacol. Sci. 25, 633.
    2. Kraft, R. et al. (2005) Pflugers. Arch. 451, 204.
    3. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690.
    4. Vazquez, E. et al. (2006) Semin. Cell Dev. Biol. 17, 607.
    Scientific background

    The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3

    The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM71,2.

    The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3

    TRPM4 and TRPM5 are the only TRP channels that are permeable to monovalent cations but not to Ca2+.

    TRPM4 is expressed as at least two alternative spliced isoforms, TRPM4a and TRPM4b which consist of shorter and longer N-terminal regions, respectively. TRPM4a displays little activity, TRPM4b and TRPM5 appear to be directly activated by an increase in intracellular Ca2+ concentration4.

    TRPM4 is expressed at relatively high levels in cardiac tissues.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-TRPM4 Antibody
    Expression of TRPM4 in various rat organs.Immunohistochemical staining of various rat organs using Anti-TRPM4 Antibody (#ACC-044) using DAB staining. TRPM4 staining (brown) is detected in rat ventricle, atrium and to a lesser extent in pancreas, kidney, liver, and lung sections.Adapted from Piao, H. et al. (2015) PLoS ONE 10, e0121703. with permission of PLoS.
    Last update: 25/10/2021

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human TRPM4. Anti-TRPM4 Antibody (#ACC-044) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize TRPM4 from human, rat, and mouse samples.

    For research purposes only, not for human use

    Applications

    Citations

    Citations
    KO validation citations
    1. Western blot analysis of mouse HL-1 cardiomyocyte lysate (1:500). Tested in siRNA treated cells.
      Son, M.J. et al. (2016) J. Physiol. 594, 2985.
    Western blot citations
    1. Human detrusor smooth muscle lysate (1:200).
      Hristov, K.L. et al. (2016) Am. J. Physiol. 310, C600.
    2. Rat atrial myocyte and mouse HL-1 cardiomyocyte lysates (1:500).
      Son, M.J. et al. (2016) J. Physiol. 594, 2985.
    3. Mouse mpkCCDc14 cell lysate (1:200).
      Wu, M.M. et al. (2016) Am. J. Physiol. 311, F1360.
    Immunohistochemistry citations
    1. Mouse brain sections.
      Riquelme, D. et al. (2018) Front. Cell. Neurosci. 12, 12.
    2. Human detrusor smooth muscle sections (1:250).
      Hristov, K.L. et al. (2016) Am. J. Physiol. 310, C600.
    3. Rat heart, kidney, and lung sections.
      Piao, H. et al. (2015) PLoS ONE 10, e0121703.
    Immunocytochemistry citations
    1. Human detrusor smooth muscle cells (1:250).
      Hristov, K.L. et al. (2016) Am. J. Physiol. 310, C600.
    2. Rat atrial myocytes (1:200).
      Son, M.J. et al. (2016) J. Physiol. 594, 2985.

    Specifications

    Scientific Background

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