Anti-TRPV4 (extracellular) Antibody

OTRPC4, VRL-2, VR-OAC, TRP12
    Cat #: ACC-124
    Alternative Name OTRPC4, VRL-2, VR-OAC, TRP12
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, r
    May also work in: m*
    Immunogen
    • Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4 (Accession Q9ERZ8). 3rd extracellular loop.
    • Anti-TRPV4 (extracellular) Antibody
    Accession (Uniprot) Number Q9ERZ8
    Gene ID 66026
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - 14/16 amino acid residues identical; mouse - 13/16 amino acid residues identical; human - 12/16 amino acid residues identical.
    RRID AB_2340915.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-TRPV4 (extracellular) Antibody
      Western blot analysis of rat brain lysate:
      1. Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:200).
      2. Anti-TRPV4 (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-TRPV4 (extracellular) Antibody
      Expression of TRPV4 in rat DRG
      Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 (extracellular) Antibody (# ACC-124). A. TRPV4 labeling appears in neuronal cell bodies. B. Nuclear staining using DAPI as counter stain. C. merged images of A and B.
    Live cell imaging / Immunocytochemistry
    • Anti-TRPV4 (extracellular) Antibody
      Expression of TRPV4 in human U-87 cells
      Cell surface detection of TRPV4 in live intact human brain glioblastoma U-87 cells. Extracellular staining of cells using  Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:25), (red) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. DAPI was used as the counterstaining (blue).
    • Anti-TRPV4 (extracellular) Antibody
      Expression of TRPV4 in human U-87 cells
      Cell surface detection of TRPV4 in live intact human brain glioblastoma U-87 cells. A. Extracellular staining of cells with Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Merged images of live cells with A.
    References
    1. Montell, C. et al. (2002) Mol. Cell. 9, 229.
    2. Clapham, D.E. (2003) Nature 426, 517.
    3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362.
    4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
    5. Gunthorpe, M.J. et al. (2002) Trends. Pharmacol. Sci. 23, 183.
    6. Peng, J.B. et al. (2003) J. Physiol. 551, 729.
    Scientific background

    TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells1. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)1-4. The TRPV subfamily consists of six members, TRPV1-65.

    TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/02/2020

    Anti-TRPV4 (extracellular) Antibody (#ACC-124) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize TRPV4 from human, rat, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Immunohistochemistry citations
    1. Rat brain sections.
      Onishi, M. et al. (2018) Physiol. Genomics 50, 272.
    2. Mouse brain sections.
      Shavit-Stein, E. et al. (2017) Front. Mol. Neurosci. 10, 42.
    Immunocytochemistry citations
    1. Human microvascular endothelial cells.
      Caires, R. et al. (2017) Cell. Rep. 21, 246.
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