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Anti-TRPV4 (extracellular) Antibody

OTRPC4, VRL-2, VR-OAC, TRP12

Cat #: ACC-124
Alternative Name OTRPC4, VRL-2, VR-OAC, TRP12
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
May also work in: m*
Immunogen
  • Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4 (Accession Q9ERZ8). 3rd extracellular loop.
Accession (Uniprot) Number Q9ERZ8
Gene ID 66026
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - 14/16 amino acid residues identical; mouse - 13/16 amino acid residues identical; human - 12/16 amino acid residues identical.
RRID AB_2340915.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, lci, wb
May also work in: ip*
Western blot
  • Western blot analysis of rat brain lysate:
    Western blot analysis of rat brain lysate:
    1. Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:200).
    2. Anti-TRPV4 (extracellular) Antibody, preincubated with TRPV4 (extracellular) Blocking Peptide (#BLP-CC124).
Immunohistochemistry
  • Expression of TRPV4 in rat DRG
    Expression of TRPV4 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 (extracellular) Antibody (# ACC-124). A. TRPV4 labeling appears in neuronal cell bodies. B. Nuclear staining using DAPI as counter stain. C. merged images of A and B.
Indirect flow cytometry
  • Cell surface detection of TRPV4 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: 
    Cell surface detection of TRPV4 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: 
    ___ Cells.
    ___  Cells + goat-anti-rabbit-FITC.
    ___ Cells +Anti-TRPV4 (extracellular) Antibody (#ACC-124), (2.5μg) + goat-anti-rabbit-FITC.
Live cell imaging / Immunocytochemistry
  • Expression of TRPV4 in human U-87 cells
    Expression of TRPV4 in human U-87 cells
    Cell surface detection of TRPV4 in live intact human brain glioblastoma U-87 cells. Extracellular staining of cells using  Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:25), (red) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. DAPI was used as the counterstaining (blue).
  • Expression of TRPV4 in human U-87 cells
    Expression of TRPV4 in human U-87 cells
    Cell surface detection of TRPV4 in live intact human brain glioblastoma U-87 cells. A. Extracellular staining of cells with Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Merged images of live cells with A.
References
  1. Montell, C. et al. (2002) Mol. Cell. 9, 229.
  2. Clapham, D.E. (2003) Nature 426, 517.
  3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362.
  4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
  5. Gunthorpe, M.J. et al. (2002) Trends. Pharmacol. Sci. 23, 183.
  6. Peng, J.B. et al. (2003) J. Physiol. 551, 729.
Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells1. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)1-4. The TRPV subfamily consists of six members, TRPV1-65.

TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 20/10/2021

Anti-TRPV4 (extracellular) Antibody (#ACC-124) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize TRPV4 from human, rat, and mouse samples.

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For research purposes only, not for human use

Applications

Citations

Citations
Immunohistochemistry citations
  1. Rat brain sections.
    Onishi, M. et al. (2018) Physiol. Genomics 50, 272.
  2. Mouse brain sections.
    Shavit-Stein, E. et al. (2017) Front. Mol. Neurosci. 10, 42.
Immunocytochemistry citations
  1. Human microvascular endothelial cells.
    Caires, R. et al. (2017) Cell. Rep. 21, 246.

Specifications

Scientific Background

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