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Anti-ZnT2 (SLC30A2) Antibody

Zinc transporter 2

Cat #: AZT-012
Alternative Name Zinc transporter 2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
  • Peptide (C)GSHPNSEKQRARRK, corresponding to amino acid residues 43-56 of rat ZnT2 (Accession Q62941). Intracellular, N-terminus.
Accession (Uniprot) Number Q62941
Gene ID 25362
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 12/14 amino acid residues identical.
RRID AB_2756812.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Will not recognize human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of mouse kidney (lanes 1 and 3) and rat small intestine (lanes 2 and 4) lysates:
    Western blot analysis of mouse kidney (lanes 1 and 3) and rat small intestine (lanes 2 and 4) lysates:
    1,2. Anti-ZnT2 (SLC30A2) Antibody (#AZT-012), (1:200).
    3,4. Anti-ZnT2 (SLC30A2) Antibody, preincubated with ZnT2/SLC30A2 Blocking Peptide (#BLP-ZT012).
  1. Lee, S. et al. (2018) Sci. Rep. 8, 3542.
  2. Lee, S. et al. (2015) J. Biol. Chem. 290,13064.
Scientific background

ZnT2 is a member of the SLC30 family of zinc transporters which promotes the influx of zinc ions into synaptic vesicles of glutamatergic neurons from the cytoplasm, intracellular organelles, or to the outside of the cell. ZnT2 is responsible for maintaining high intravesicular zinc content. In addition, ZnT2 also imports zinc into the mitochondria, which constricts ATP generation and activates apoptosis. ZnT2 is encoded by the Slc30a2 gene and functions as a homo- or heterodimer. The protein structure consists of six transmembrane domains with cytoplasmic N- and C-termini that contain multiple regulatory domains1.

Expression of ZnT2 is restricted to secretory cells, such as acinar pancreatic cells, prostate epithelial cells, placental trophoblasts, placenta, ovary and mammary epithelial cells1,2.

Studies show that eight missense mutations have been identified in the human SLC30A2 gene that lead to pathologically low breast milk Zn concentrations and severe zinc deficiency in breastfed infants, a disorder known as transient neonatal zinc deficiency1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/03/2021

Anti-ZnT2 (SLC30A2) Antibody (#AZT-012) is a highly specific antibody directed against an epitope of the rat Zinc transporter 2. The antibody can be used in western blot analysis. It has been designed to recognize ZnT2 from mouse and rat samples. It will not recognize the protein from human samples.

For research purposes only, not for human use
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