Cono VGRPRF-amide

A Specific Potentiator of ASIC3 Channel
Cat #: STC-172
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Conus textile.
    Source Synthetic peptide
    MW: 730 Da.
    Purity: >98% (HPLC)
    Form Lyophilized powder.
    Effective concentration 50 µM.
    Sequence VGRPRF.
    Modifications C-terminal amidation at Phe6.
    Molecular formula C33H55N13O6.
    Activity Cono VGRPRF-amide is an ASIC3 channel positive modulator1.
      • Reimers, C. et al. (2017) Proc. Natl. Acad. Sci. U. S. A. 114, E3507.

    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g for 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • Alomone Labs Cono VGRPRF-amide positively modulates ASIC3 channels expressed in Xenopus oocytes.
        A. Representative time course of Cono VGRPRF-amide (#STC-172) potentiation of ASIC3 current. Membrane potential was held at -80 mV, current was elicited every 100 sec by a transient application of pH 5.5, and reversibly potentiated by a 60 sec application of 50 µM Cono VGRPRF-amide, as indicated. B. Superimposed traces of ASIC3 currents before (black) and after (green) application of 50 µM Cono VGRPRF-amide, which significantly extends the current following stimulation (arrow). Taken from the experiment in A.
        Alomone Labs Cono RF-amide peptides positively modulate ASIC3 channels expressed in Xenopus oocytes.
        Representative superimposed traces of ASIC3 currents before (black) and following a 60 sec application of 50 µM Cono RPRF-amide (#STC-170), Cono VGRPRF-amide (#STC-172) or Cono AIVGRPRF-amide (#STC-174) (green), which significantly extend the current evoked by a transient application of pH 5.5 (arrow), at membrane potential of -80 mV.
      • Reimers, C. et al. (2017) Proc. Natl. Acad. Sci. U. S. A. 114, E3507.
      • Osmakov, D.I. et al. (2017) Front. Mol. Neurosci. 10, 282.
      • Cono VGRPRF-amide conotoxin is a peptide toxin originally isolated from the venom of the cone snail Conus textile and strongly potentiates ASIC3 by slowing the desensitization kinetics of the channel. Cono VGRPRF-amide belongs to the subgroup of cono-RFamides containing C-terminal sequence of four amino acids and a C-terminal Arg–Phe–NH2 (RFa) motif1.

        ASIC channels are proton-gated Na+ channels directly activated by extracellular protons. They are expressed throughout the CNS and the peripheral nervous system. They are responsible for synaptic transmission and detection of painful acidosis. ASICs play an important role in several physiological and pathological conditions including synaptic plasticity and learning, fear conditioning, and neurodegeneration associated with ischemic stroke1,2.

    Target ASIC3 channel
    Net Peptide Content: 100%
    Last update: 23/01/2019

    Cono VGRPRF-amide (#STC-172) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use