Every lot is tried & tested in a relevant biological assay.
- Alomone Labs Fasciculin-I potently inhibits Acetylcholinesterase (AChE) activity.AChE activity was determined colorimetrically using Ellman's reagent, as production of thiocholine from acetylthiocholine. Absorbance was measured at 405 nm after a 30 min incubation. Normalized activity of 400 mU/ml human AChE, as per cent of control, was plotted against increasing concentrations of Fasciculin-I (#F-650), showing concentration-dependent inhibition with pIC50 ≈ 6.7.
Fasciculin-I is a 61 amino acid peptidyl toxin with four disulfide bonds, isolated from the Eastern green mamba (Dendroaspis angusticeps) venom. Fasciculin-I belongs to the three finger toxin family, structurally similar to the short alpha-neurotoxins and cardiotoxins1.
Fasciculin-I was shown to be a selective, potent, and reversible blocker of the mammalian acetylcholinesterase (AChE) at picomolar concentrations1. Injection of Fasciculin-I to mice causes severe generalized and long-lasting twitch (fasciculation) due to its ability to accumulate acetylcholine at the neuromuscular junction2.
Fasciculin-I (#F-650) is a highly pure, natural, and biologically active peptide toxin.