- Peptide CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1 (Accession Q14246). Extracellular, N-terminus.
- Human promyelocytic leukemia (HL-60), human Burkitt's lymphoma (Raji) B, mouse macrophage (J774), rat basophilic leukemia (RBL) cell lysates; rat and mouse fat and spleen tissues (1:200-1:1000).
- Western blot analysis of human promyelocytic leukemia (HL-60) (lanes 1 and 5), human Burkitt's lymphoma (Raji) B (lanes 2 and 6), mouse macrophage J774 (lanes 3 and 7) and rat basophilic leukemia (RBL) (lanes 4 and 8) cell lysates:1-4. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051-GP), (1:200).
5-8. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with EMR1/ADGRE1 (extracellular) Blocking Peptide (#BLP-ER051).
Human epidermal growth factor (EGF)–like module containing mucin-like hormone receptor 1 (EMR1) is a surface receptor with unknown function that belongs to the adhesion family of the G protein-coupled receptors (GPCR). This receptor is encoded by the ADGRE1 and like all GPCRs, contains seven transmembrane domains, an intracellular C-terminus and an extracellular N-terminus. The NH2 domain has six EGF-like domains.
Adhesion GPCRs share a long N-terminal extracellular sequences comprising multiple domains, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties1,2.
EMR1 protein is a homolog of F4/80, a murine protein that is widely being used as a marker of murine macrophage populations1.
Human EMR1 is exclusively expressed on mature eosinophils in the blood and bone marrow and in nasal polyps. Thus, EMR1 is considered to be a potential therapeutic target for the treatment of eosinophilic disorders1,3.