Overview
- Peptide (C)RDAIVKVKEVHDE, corresponding to amino acid residues 183-195 of rat Gephyrin (Accession Q03555). Intracellular.
Gephyrin Blocking Peptide (#BLP-IP005)
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Guinea Pig Anti-Gephyrin Antibody (#AIP-005-GP), (1:200).
3-4. Guinea Pig Anti-Gephyrin Antibody (#AIP-005-GP), preincubated with Gephyrin Blocking Peptide (#BLP-IP005).
Expression of Gephyrin in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-Gephyrin Antibody (#AIP-005-GP), (1:1000), followed by donkey anti-guinea pig-biotin and Streptavidin-Cy3. A. Gephyrin showed immunoreactivity (red) in the mossy fiber terminal zone (MFT, arrow) and in the pyramidal layer of the CA3 region (CA3). B. Pre-incubation of the antibody with Gephyrin Blocking Peptide (#BLP-IP005), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Chen, J. et al. (2014) Front. Cell. Neurosci. 8, 276.
- Tyagarajan, S.K. and Fritschy, J.M. (2010) J. Physiol. 588, 101.
- Maric, H.M. et al. (2014) ACS Chem. Biol. 9, 2554.
- Fritschy, J.M. et al. (2008) Trends Neurosci. 31, 257.
- Zacchi, P. et al. (2014) Front. Cell. Neurosci. 8, 103.
- Lorenzo, L.E. et al. (2014) J. Neurosci. 34, 8300.
Gephyrin is a highly conserved, intracellular scaffolding protein responsible for the post-synaptic anchoring and assembly of the inhibitory glycine and γ-aminobutyric acid type A receptors (GlyR and GABAAR); it essentially anchors the receptors, by auto-oligomerization, to the cytoskeleton1,2.
GABAergic transmission is important for the mediation of neuronal excitability and has a crucial role in neurodevelopment, throughout the central and peripheral nervous systems (especially via GABAAR). Glycinergic neurons on the other hand control motor functions and sensory signaling as well as take part in pain transmission in the spine's dorsal horn. Importantly, gephyrin has a 1:1 stoichiometry with GlyR3,4.
Up-to-date atomic force microscopy data of gephyrin has shown that it forms trimers with multiple conformations. It is a modular construct with three domains: an N-terminal G-, a central C- and a C-terminal E-domain. Recent work suggests also that gephyrin regulates neuronal differentiation by anchoring small cytoskeletal-related signaling molecules at unique subcellular locations2,5,6.
Guinea Pig Anti-Gephyrin Antibody (#AIP-005-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Gephyrin from mouse, rat and human samples.
The antigen used to immunize guinea pigs is the same as Anti-Gephyrin Antibody (#AIP-005) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.
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