Guinea pig Anti-Periaxin Antibody

PRX, CMT4F
  • New
Cat #: AGP-215
Alternative Name PRX, CMT4F
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Guinea Pig
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)DVEFSFPKFSRLRR, corresponding to amino acid residues 155-170 of rat PRX (Accession Q63425). Intracellular, N-terminus.
    • Guinea pig Anti-Periaxin Antibody
    Accession (Uniprot) Number Q63425
    Gene ID 78960
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Guinea pig total IgG
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    • Guinea pig Anti-Periaxin Antibody
      Western blot analysis of rat DRG lysate:
      1. Guinea pig Anti-Periaxin Antibody (#AGP-215), (1:200).
      2. Guinea pig Anti-Periaxin Antibody, preincubated with the negative control antigen.
    • Guinea pig Anti-Periaxin Antibody
      Western blot analysis of mouse lung lysate:
      3. Guinea pig Anti-Periaxin Antibody (#AGP-215), (1:600).
      4. Guinea pig Anti-Periaxin Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Guinea pig Anti-Periaxin Antibody
      Expression of Periaxin in rat spinal dorsal root entry zone
      Immunohistochemical staining of rat spinal dorsal root entry zone frozen sections using Guinea pig Anti-Periaxin Antibody (#AGP-215). Periaxin immunoreactivity (red) appears in the dorsal nerve root (arrows). Nuclei are stained using DAPI as the counterstain (blue).
    References
    1. Williams, C. and Brophy, P.J. (2002) J. Anat. 200, 323.
    2. Han, H. and Kursula, P. (2014) J. Biol. Chem289, 14121.
    3. Shi, Y. et al. (2014) PLoS One. 9, 91953.
    Scientific background

    Periaxin protein is a membrane-associated protein expressed in myelinating Schwann cells. It is required for the stable maintenance and development of a normal myelin sheath in the peripheral nervous system. It is also a member of cytoskeletal complexes in lens fibers where it plays an important role in maturation, packing, and membrane organization of lens fiber cell1,2

    The periaxin gene (PRX) encodes two protein isoforms, L-periaxin and S-periaxin generated by alternative mRNA splicing. These proteins contain N-terminal PDZ domains (PSD-95/Discs-large/ZO-1) important for organizing protein–protein interactions.

    L-periaxin is a component of the dystroglycan–dystrophin-related protein-2 complex (DRP2) linking the Schwann cell cytoskeleton to the extracellular matrix. Its location changes during myelination; at first the protein is localized to the adaxonal plasma membrane and later, in the abaxonal plasma membrane. S-periaxin is distributed in a uniform manner in the cytoplasm and the nucleus of the Schwann cell1-3

    Mutations in the Periaxin gene are known to cause autosomal recessive demyelinating in Type 4F Charcot-Marie-Tooth (CMT4F) and Dejerine-Sottas disease3.

    Periaxin knockout mice develop normally myelinated peripheral nervous system at first but develop a late onset demyelinating peripheral neuropathy1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 19/02/2020

    Guinea pig Anti-Periaxin Antibody (#AGP-215), raised in guinea pig, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Periaxin from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Periaxin Antibody (#ANR-091) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use
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