Overview
- Peptide (C)EFRQEEFRKLAGR, corresponding to amino acid residues 2-14 of rat VGLUT1 (Accession Q62634). Intracellular, N-terminus.
VGLUT1 Blocking Peptide (#BLP-GC035)
Western blot analysis of rat brain cortex lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Guinea Pig Anti-VGLUT1 Antibody (#AGC-035-GP), (1:400).
3-4. Guinea Pig Anti-VGLUT1 Antibody, preincubated with VGLUT1 Blocking Peptide (#BLP-GC035).
Expression of VGLUT1 in mouse dorsal root nucleus (DRN).Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea Pig Anti-VGLUT1 Antibody (#AGC-035-GP), (1:300), followed by goat anti-guinea pig-Alexa Fluor-594. A. VGLUT1 immunoreactivity (red) appears in in DRN cells (arrows). B. Pre-incubation of the antibody with VGLUT1 Blocking Peptide (#BLP-GC035), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of VGLUT1 in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Guinea Pig Anti-VGLUT1 Receptor 1 Antibody (#AGC-035-GP), (1:300), followed by goat anti-guinea pig-Alexa Fluor-594. A. VGLUT1 immunoreactivity (red) appears in layers 1-2 of spinal dorsal horn (vertical arrows) and in the dorsal root (horizontal arrow). B. Pre-incubation of the antibody with VGLUT1 Blocking Peptide (#BLP-GC035), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Li, T. et al. (2005) World. J. Gastroenterol. 11, 1731.
- Foss, S.M. et al. (2013) J. Neurosci. 33, 10647.
- King, M.V. et al. (2014) Neuropsychopharmacology. 39, 464.
Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system (CNS) and is responsible for most of the fast synaptic neurotransmission. Glutamate has great contribution for learning, memory, and neuronal plasticity. Dysregulation of glutamate transmission plays a role in the pathophysiology of several psychiatric and neurological diseases1.
Three vesicular glutamate transporters (VGLUT1, VGLUT2, and VGLUT3) have been identified. They are responsible for packaging and transporting glutamate into synaptic vesicles. VGLUTs in part regulate the storage and release of glutamate in excitatory circuits in the brain.
All three VGLUT isoforms are highly conserved and have the same predicted topology including 12 putative transmembrane domains. They all share a conserved dileucine-like motif in the C terminus. This motif was found to be important for VGLUT1 endocytosis. In addition, VGLUT1 contains two polyproline domains distal to the C-terminal dileucine-like motif, including one that interacts with the endocytic BAR (Bin/Amphiphysin/Rvs) domain protein endophilin. Two additional dileucine-like motifs are present in the N terminus of VGLUT1 that are not well conserved in the other isoforms1-3.
VGLUT1 is expressed in the hippocampus, cerebral cortex, and cerebellar cortex and appears to be the main isoform expressed in the cortex. The transporter is also detected in the enteric nervous system and pancreatic tissue. VGLUT1 has been suggested to influence the properties of the release of glutamate1,2.
Guinea Pig Anti-VGLUT1 Antibody (#AGC-035-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize VGLUT1 from mouse, rat and human samples.
The antigen used to immunize guinea pigs is the same as Anti-VGLUT1 Antibody (#AGC-035) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.
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