Certificate of Analysis

hERG Related Channel Modulator Explorer Kit

A Screening Package of hERG Related Channel Modulators Economically Priced
Cat #: EK-109 12 Vials

BeKm-1 Cat #: STB-470

Size: 0.1 mg MW: 4092 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
2-10 nM.
Activity
BeKm-1 inhibits ERG1 K+ channel currents and has minimal effect on ELK1 K+ channels.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Dofetilide Cat #: D-100

Size: 5 mg Source: Synthetic MW: 441.6 Purity: >98%
Effective concentration
0.5-100 μM.
Activity
Dofetilite is a class II antiarrhythmic agent, which blocks the fast delayed rectifier cardiac potassium current, mostly carried out by the KV11.1 (hERG) channels1. Dofetilide was effective in inhibiting KV11.1 channels in Xenopus oocytes with 1 μM, thereby eliminating the current2. 500 nM Dofetilide enhanced smooth muscle from bovine epididymal duct contractions as expected by blocking KV11.1 channels3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

E-4031 Cat #: E-500

Size: 0.5 mg Source: Synthetic MW: 510.5 Purity: >98%
Effective concentration
100 nM - 1 μM.
Activity
E-4031 is a methsnesulfonanilide class III antiarrhythmic agent. It specifically blocks hERG (KV11.1) voltage-gated K+ channel current in heterologous systems1,2, cardiac cells3 and cardiac preparations4.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or six months at -20°C.

Ergtoxin-1 Cat #: STE-450

Size: 0.1 mg MW: 4730 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
10-100 nM.
Activity
Ergtoxin-1 is a potent inhibitor of ERG K+ channels of nerve, heart and endocrine cells of different species1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

GrTx1 Cat #: STG-250

Size: 50 µg MW: 3697 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
20-500 nM.
Activity
GrTx1 is a blocker of voltage-gated Na+ channels and herg1 channels1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

GsAF-I Cat #: STG-300

Size: 50 µg MW: 3707.9 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
40 nM – 1.3 µM.
Activity
GsAF-I is a blocker of TTX-sensitive NaV and hERG1 channels1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

GsAF-II Cat #: STG-350

Size: 50 µg MW: 3979.9 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1-24 µM.
Activity
GsAF-II is a blocker of TTX-sensitive NaV and KV11.1 channels1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Haplotoxin-2 Cat #: STT-200

Size: 50 µg MW: 3708 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
100-500 nM.
Activity
Haplotoxin-2 inhibits voltage-gated Na+ channels NaV1.3 as well as KV11.1 (hERG) channels1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
20 mM ammonium acetate or any other aqueous buffer, pH > 8. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Miconazole nitrate Cat #: M-206

Size: 1 g Source: Synthetic MW: 479.1 Purity: >97%
Effective concentration
1-100 µM.
Activity
Miconazole blocks hERG in HEK transfected cells as well as KV1.3 and KV1.51.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

NS-1643 Cat #: N-115

Size: 10 mg Source: Synthetic MW: 380.2 Purity: >95%
Effective concentration
1-100 μM.
Activity
NS-1643 is a potent and selective KV11.1 (hERG, ERG1, erg1) channel modulator (enhancer)1. NS-1643 was effective in eliciting and enhancing KV11.1 channels in Xenopus oocytes with apparent EC50 of 20 μM1. NS-1643 inhibited smooth muscle from bovine epididymal duct contractions with EC50 values of 8 μM2. It also potentiates KCNQ2 but not KCNQ1 channels3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

NS-3623 Cat #: N-190

Size: 5 mg Source: Synthetic MW: 427.18 Purity: >95%
Effective concentration
10-200 µM.
Activity
NS-3623 is an opener of KV11.1 channels with an EC50 of 10.5 μM1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Trimethylapigenin Cat #: T-145

Size: 5 mg Source: Synthetic MW: 312.3 Purity: >96%
Effective concentration
1-200 µM.
Activity
Trimethylapigenin blocks KV1.5 and hERG channels with IC50 ~ 8 and 30 μM respectively. It also activates CFTR channels1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
50 mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

For more information, please refer to individual certificate of analysis for each product.