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Certificate of Analysis

Intracellular Ca2+ Regulator Antibody Explorer Kit

A Screening Package of Intracellular Ca2+ Regulator Antibodies Economically Priced
Cat #: AK-455
Size: 13 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-IP3 Receptor-1 (ITPR1) Antibody Cat #: ACC-019

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)RIGLLGHPPHMNVNPQQPA, corresponding to amino acid residues 2732-2750 of rat IP3R1 (Accession P29994). Intracellular, C-terminus.
Homology
Human, mouse - identical.

Anti-IP3 Receptor-2 (ITPR2) Antibody Cat #: ACC-116

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: R
Immunogen
Peptide (C)RLGFLGSNTPHENH, corresponding to amino acid residues 2683-2696 of rat IP3R2 (Accession P29995). Intracellular, C-terminus.
Homology
Mouse, human - 13/14 amino acid residues identical.

Anti-MCUR1 (CCDC90A) Antibody Cat #: ACC-321

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)GRNALGRLRLGARR, corresponding to amino acid residues 97-110 of mouse mitochondrial calcium uniporter regulator 1 (Accession Q9CXD6). Intracellular loop, mitochondrial matrix.
Homology
Rat - identical; human – no apparent homology.

Anti-MCU Antibody Cat #: ACC-328

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide CIEQHQLNKERELIER, corresponding to amino acid residues 191-206 of human mitochondrial calcium uniporter (Accession Q8NE86). N-terminus, mitochondrial matrix.
Homology
Rat, mouse – 15/16 amino acid residues identical.

Anti-MICU1 Antibody Cat #: ACC-322

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)EFERHDPVDGRISER, corresponding to amino acid residues 313-327 of rat MICU1 (Accession Q6P6Q9). Intracellular.
Homology
Mouse - identical; human - 14/15 amino acid residues identical.

Anti-Orai1 (extracellular) Antibody Cat #: ACC-062

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)KFLPLKRQAGQPS, corresponding to amino acid residues 200-212 of mouse Orai1 (Accession Q8BWG9). 2nd extracellular loop.
Homology
Rat - identical.

Anti-Human Orai1 (extracellular) Antibody Cat #: ACC-060

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, WB Reactivity: H
Immunogen
Peptide (C)KKQPGQPRPTSK, corresponding to amino acid residues 203-214 of human Orai1 (Accession Q96D31). 2nd extracellular loop.
Homology
Canis - identical; bovine - 12/13 amino acid residues identical.

Anti-Ryanodine Receptor 1 Antibody Cat #: ARR-001

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)RAENEKDATTEKNKKR, corresponding to amino acid residues 1371-1386 of human RyR1 (Accession P21817). Intracellular, N-terminus.
Homology
Rat, mouse - identical.

Anti-Ryanodine Receptor 2 Antibody Cat #: ARR-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide CAGESMSPGQGRNN, corresponding to amino acid residues 1489-1502 of human RyR2 (Accession Q92736). Intracellular, N-terminus.
Homology
Rat, mouse - identical.

Anti-Ryanodine Receptor 3 Antibody Cat #: ARR-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide CHIAHVNPAAEEDLK, corresponding to amino acid residues 935-949 of human RyR3 (Accession Q15413). Intracellular, N-terminus.
Homology
Rat, mouse - identical.

Anti-SERCA1 Antibody Cat #: ACP-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)TPDQVKRHLEKYG, corresponding to amino acid residues 25-37 of rat SERCA1 (Accession Q64578). Cytoplasmic, N-terminus.
Homology
Mouse - identical; human - 12/13 amino acid residues identical.

Anti-SERCA2 Antibody Cat #: ACP-012

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, WB Reactivity: H, M, R
Immunogen
Peptide CTPNKPSRTSMSK, corresponding to amino acid residues 498-510 of rat SERCA2 (Accession P11507). 2nd cytoplasmic loop.
Homology
Mouse, human - identical.

Anti-STIM1 (extracellular) Antibody Cat #: ACC-063

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 of human STIM1 (Accession Q13586). STIM1 expressed on the plasma membrane: Extracellular, N-terminus. STIM1 expressed on the ER: Luminal, N-terminus.
Homology
Rat, mouse, bovine, canis - identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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