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Maurocalcine

MCa, Maurocalcin

A Ryanodine Receptor 1, 2 & 3 Modulator

Cat #: RTM-100
Alternative Name MCa, Maurocalcin
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Recombinant, E. coli
    MW: 3864 Da.
    Purity: >98% (HPLC)
    Effective concentration 200-400 nM.
    Sequence GDCLPHLKLCKENKDCCSKKCKRRGTNIEKRCR.
    Modifications Disulfide bonds between Cys3-Cys17, Cys10-Cys21 and Cys16-Cys32.
    Structure
    Molecular formula C156H260N56O46S6.
    Activity Maurocalcine is an agonist of Ryanodine receptor channel (RyR) types 1‚ 2 and 3.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs Maurocalcine evokes long lasting Ca2+ release events in skeletal myotubes.
      Alomone Labs Maurocalcine evokes long lasting Ca2+ release events in skeletal myotubes.
      Myotube cell cultures were prepared from limbs of 2 days old rat pups, were loaded with 4 mM fluo-3 AM and then stimulated with Maurocalcine (#RTM-100). Ca2+ spreads in the same myotube before (A) and after (B) stimulation with 200 nM Maurocalcine.
    • Alomone Labs Maurocalcine prolongs the duration of spontaneous long lasting Ca2+ release events in cardiomyocytes.
      Alomone Labs Maurocalcine prolongs the duration of spontaneous long lasting Ca2+ release events in cardiomyocytes.
      High density cardiomyocyte cell culture prepared from hearts of 3-4 day old rat pups were used 3 days after seeding. The cells were loaded with 4 mM fluo-3 AM and the Ca2+ traces were measured before (blue) and after (red) stimulation with 400 nM Maurocalcine (#RTM-100).
    References - Scientific background
    1. Fajloun, Z. et al. (2000) FEBS Lett469, 179.
    2. Mosbah, A. et al. (1998) 6eme Rencontre en Toxinologie,  Société française pour l'étude des toxines, Paris. August 1-4.
    3. Estève, E. et al. (2005) J. Biol. Chem280, 12833.
    4. Chen , L. et al. (2003) J. Biol. Chem278, 16095.
    5. Tripathy, A. et al. (1998) J. Gen. Physiol111, 679.
    Scientific background

    Maurocalcine (MCa) is a peptidyl toxin originally isolated from the venom of the scorpion Scorpio maurus palmatus.

    Native MCa is present at a low concentration in the venom (0.5% of total protein). This 33-mer basic peptide contains three disulfide bridges and has 82% sequence homology to Imperatoxin A (IpTxa), a scorpion toxin from the venom of Pandinus imperator. MCa shares the highly basic structural domain identified in peptide A - a segment of the dihydropyridine receptor and IpTxa which is important for interactions with ryanodine receptors. MCa also appears to share the same features as the so-called cell-penetrating peptides which allows the toxin to efficiently penetrate various cell types without expending metabolic energy or using an endocytotic mechanism.1-4

    In vitro, electrophysiological experiments based on recordings of single channels incorporated into planar lipid bilayers showed that MCa potently and reversibly modifies channel gating behavior of the ryanodine receptor 1 (RyR1) by inducing prominent subconductance behavior.1,2

    Similar to IpTxa, MCa-modified channels can reversibly shuttle between subconductance states and fast gating states. The actions of these peptides were also found to be additive with those of ryanodine, resulting in additional sub-states along with the ryanodine-locked half-conducting state. However, the predominant subconductance induced by MCa differs from that of IpTxa. At a holding potential of +40 mV, the predominant substates induced by sMCa and IpTxa are 48 and 28% of the full conductance, respectively, suggesting that the structural difference in these peptides may induce slightly different channel conformations that stabilize different unitary conductances.5

    Target Ryanodine receptors
    Net Peptide Content: 100%
    Last update: 24/01/2021

    Maurocalcine (#RTM-100) is a highly pure, recombinant, and biologically active peptide toxin.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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