Certificate of Analysis

Monoamine Transporter Antibody Explorer Kit

A Screening Package of Monoamine Transporter Antibodies Economically Priced
Cat #: AK-445 10 Vials

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-Dopamine Transporter (DAT) (extracellular) Antibody Cat #: AMT-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)DAHASNSSDGLGLND, corresponding to amino acids residues 191-205 of rat DAT (Accession P23977). 2nd extracellular loop.
Homology
Mouse - 14/15 amino acid residues identical.

Anti-Noradrenaline Transporter (NET) (extracellular) Antibody Cat #: AMT-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)KLLNASVLGDHTKYSK, corresponding to amino acid residues 189-204 of mouse NET (Accession O55192). 2nd extracellular loop.
Homology
Rat - identical; human - 14/16 amino acid residues identical.

Anti-Serotonin Transporter (SERT) (extracellular) Antibody Cat #: AMT-004

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)EMRNEDVSEVAKD, corresponding to amino acids residues 388-400 of rat SERT (Accession P31652). 4th extracellular loop.
Homology
Mouse, human - identical.

Anti-VMAT1 Antibody Cat #: AMT-007

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)EFKDSNSSLHRGPS, corresponding to amino acid residues 53-66 of rat VMAT1 (Accession Q01818). 1st luminal loop.
Homology
Mouse - 12/14 amino acid residues identical.

Anti-VMAT2 Antibody Cat #: AMT-006

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)KHEKNSTEIQT(A)R, corresponding to amino acid residues 52-64 of rat VMAT2 (Accession Q01827). 1st luminal loop.
Homology
Rat, mouse, human – 12/13 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.