Overview
Cat #: BLP-NR052
Type: Synthetic peptide
Form: Lyophilized powder
Nectin-2/PVRL2 (extracellular) Blocking Peptide (#BLP-NR052) is the original antigen used for immunization during Anti-Nectin-2/PVRL2 (extracellular) Antibody (#ANR-052) generation. The blocking peptide binds and ‘blocks’ Anti-Nectin-2/PVRL2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat spleen lysate (lanes 1 and 4), human Jurkat T cell leukemia cell lysate (lanes 2 and 5) and human K562 erythroleukemia cell lysate (lanes 3 and 6):1-3. Anti-Nectin-2/PVRL2 (extracellular) Antibody (#ANR-052), (1:200).
4-6. Anti-Nectin-2/PVRL2 (extracellular) Antibody, preincubated with Nectin-2/PVRL2 (extracellular) Blocking Peptide (#BLP-NR052). - Expression of Nectin-2 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Nectin-2/PVRL2 (extracellular) Antibody (#ANR-052), (1:600), followed by goat-anti-rabbit-AleaxaFluor-488. Nectin-2 staining (green) appears in in apical dendrites of CA1 pyramidal neurons (vertical arrows) and in astrocytes (horizontal arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
Accession (Uniprot) Number P32507
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.