Nectin-2/PVRL2 (extracellular) Blocking Peptide (#BLP-NR052) is the original antigen used for immunization during Anti-Nectin-2/PVRL2 (extracellular) Antibody (#ANR-052) generation. The blocking peptide binds and ‘blocks’ Anti-Nectin-2/PVRL2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat spleen lysate (lanes 1 and 4), human Jurkat T cell leukemia cell lysate (lanes 2 and 5) and human K562 erythroleukemia cell lysate (lanes 3 and 6):
- Expression of Nectin-2 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Nectin-2/PVRL2 (extracellular) Antibody (#ANR-052), (1:600), followed by goat-anti-rabbit-AleaxaFluor-488. Nectin-2 staining (green) appears in in apical dendrites of CA1 pyramidal neurons (vertical arrows) and in astrocytes (horizontal arrows). Cell nuclei are stained with DAPI (blue).
- (C)RVIAQPENHAEAQE, corresponding to amino acid residues 148-161 of mouse Nectin-2 (Accession P32507).