Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nectin-2/PVRL2 (extracellular) Blocking Peptide (#BLP-NR052) is the original antigen used for immunization during Anti-Nectin-2/PVRL2 (extracellular) Antibody (#ANR-052) generation. The blocking peptide binds and ‘blocks’ Anti-Nectin-2/PVRL2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat spleen lysate (lanes 1 and 4), human Jurkat T cell leukemia cell lysate (lanes 2 and 5) and human K562 erythroleukemia cell lysate (lanes 3 and 6):1-3. Anti-Nectin-2/PVRL2 (extracellular) Antibody (#ANR-052), (1:200).
4-6. Anti-Nectin-2/PVRL2 (extracellular) Antibody, preincubated with Nectin-2/PVRL2 (extracellular) Blocking Peptide (#BLP-NR052).
Expression of Nectin-2 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Nectin-2/PVRL2 (extracellular) Antibody (#ANR-052), (1:600), followed by goat-anti-rabbit-AleaxaFluor-488. Nectin-2 staining (green) appears in in apical dendrites of CA1 pyramidal neurons (vertical arrows) and in astrocytes (horizontal arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RVIAQPENHAEAQE, corresponding to amino acid residues 148-161 of mouse Nectin-2 (Accession P32507).
Accession (Uniprot) Number P32507
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
