Overview
Nicotinic Acetylcholine Receptor α1/CHRNA1 (extracellular) Blocking Peptide (#BLP-NC001) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody (#ANC-001) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor α1/CHRNA1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications
Western blot analysis of rat skeletal muscle (lanes 1 and 3) and mouse C2C12 myoblasts (lanes 2 and 4):1,2. Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody (#ANC-001), (1:200).
3,4. Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α1/CHRNA1 (extracellular) Blocking Peptide (#BLP-NC001).
Properties
- EHETRLVAKLFKD(C), corresponding to amino acid residues 22-34 of rat nAChRα1 (Accession P25108).
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