Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nicotinic Acetylcholine Receptor α1/CHRNA1 (extracellular) Blocking Peptide (#BLP-NC001) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody (#ANC-001) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor α1/CHRNA1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat skeletal muscle (lanes 1 and 3) and mouse C2C12 myoblasts (lanes 2 and 4):1,2. Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody (#ANC-001), (1:200).
3,4. Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α1/CHRNA1 (extracellular) Blocking Peptide (#BLP-NC001).
Properties
Sequence
- EHETRLVAKLFKD(C), corresponding to amino acid residues 22-34 of rat nAChRα1 (Accession P25108).
Accession (Uniprot) Number P25108
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
