Overview
Cat #: BLP-NC007
Type: Synthetic peptide
Form: Lyophilized powder
Nicotinic Acetylcholine Receptor α7/CHRNA7 (extracellular) Blocking Peptide (#BLP-NC007) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor α7/CHRNA7 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain cell lysates:1,2. Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007), (1:200).
3,4. Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α7/CHRNA7 (extracellular) Blocking Peptide (#BLP-NC007). - Expression of nAChRα7 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007), (1:200). nAChRα7 (red staining) is expressed in DRG neurons. Hoechst 33342 is used as the counterstain (blue).
Properties
Sequence
Accession (Uniprot) Number Q05941
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.