Overview
Nicotinic Acetylcholine Receptor β4/CHRNB4 (extracellular) Blocking Peptide (#BLP-NC014) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody (#ANC-014) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor β4/CHRNB4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications
Western blot analysis of SH-SY5Y human neuroblastoma cell lysate (lanes 1 and 4), rat dorsal root ganglion (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates:1-3. Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody (#ANC-014), (1:200).
4-6. Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor β4/CHRNB4 (extracellular) Blocking Peptide (#BLP-NC014).
Expression of nAChR β4 in rat dorsal root ganglionImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody (#ANC-014). A. nAChRβ4 labeling (red) appears in neuronal cell bodies. B. Nuclear staining using DAPI as counterstain (blue). C. Merged images of A and B.
Properties
- CYEGVNILRIPAKR, corresponding to amino acid residues 95-108 of rat nAChRβ4 (Accession P12392).
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