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Certificate of Analysis

Nucleoside Transporter Antibody Explorer Kit

A Screening Package of Nucleoside Transporter Antibodies Economically Priced

Cat #: AK-450
Size: 5 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-ENT1 (SLC29A1) Antibody Cat #: ANT-051

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, R
Immunogen
Peptide (C)EQETKLDLISKGEEPR, corresponding to amino acid residues 245-260 of human ENT1 (Accession Q99808). 3rd intracellular loop.
Homology
Mouse, rat - 15/16 amino acid residues identical.

Anti-ENT2 (SLC29A2) Antibody Cat #: ANT-052

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)EPQKPGKPSVFVVFRK, corresponding to amino acid residues 273-288 of rat ENT2 (Accession O54699). 3rd intracellular loop.
Homology
Mouse - identical; human - 14/16 amino acid residues identical.

Anti-ENT3 (SLC29A3) Antibody Cat #: ANT-053

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)DYPAPGLQRPEDR, corresponding to amino acid residues 37-49 of rat ENT3 (Accession Q80WK7). Intracellular, N-terminus.
Homology
Mouse – identical; human – 10/13 amino acid residues identical.

Anti-SLC28A1 (CNT1) Antibody Cat #: ANT-061

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)ENMGAEFLESMEEGR, corresponding to amino acid residues 22-36 of rat SLC28A1 (Accession Q62674). Intracellular, N-terminus.
Homology
Mouse - 13/15 amino acid residues identical; human - 12/15 amino acid residues identical.

Anti-SLC28A2 (CNT2) Antibody Cat #: ANT-062

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)EVTQGHSLKDGLGH, corresponding to amino acid residues 40-53 of rat SLC28A2 (Accession Q62773). Intracellular, N-terminus.
Homology
Mouse - 13/14 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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