A Mitochondrial ATP Synthase Inhibitor
    Cat #: O-500
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Streptomyces diastatochromogenes.
    Source Natural
    MW: 791.06
    Purity: >99%
    Effective concentration 0.1-100 µM.
    • Oligomycin
    Chemical name (1S,4E,5'R,6R,6'R,7S,8R,10S,11S,12R,14S,15R,16S,18E,20E,22S,25R,27S,28R,29S)-22-ethyl-7,11,14,15-tetrahydroxy-6'- [(2S)-2-hydroxypropyl]-5',6,8,10,12,14,16,28,29-nonamethylspiro[2,26- dioxabicyclo[23.3.1]nonacosa-4,18,20-triene-27,2'-oxane] -3,9,13-trione (for Olygomycin A).
    Molecular formula C45H74O11 (for Oligomycin A).
    CAS No.: 1404-19-9.
    Activity Oligomycin, a natural antibiotic, inhibits mitochondrial H+-ATP synthase. It is commonly used to inhibit oxidative phosphorylation1,2.
    1. Chappell, J.B. and Greville, G.D. (1961) Nature 190, 502.
    2. Kagawa, Y. and Racker, E. (1966) J. Biol. Chem241, 2461.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility DMSO, ethanol, acetone. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or six months at -20°C.
    Our bioassay
    • Oligomycin
      Alomone Labs Oligomycin inhibits ATP-dependent adipogenesis in 3T3-L1 cells.
      Cells were induced to undergo adipocyte differentiation (A-D) for 5 days. Different concentrations of Oligomycin (#O-500) were then added (B, 1 µg/ml; C, 2 µg/ml and D, 10 µg/ml). After 10 days, the cells were stained with Oil Red O and visualized under light microscopy.
    Note Oligomycin is a mixture of A, B and C isomers.
    References - Scientific background
    1. Chappell, J.B. and Greville, G.D. (1961) Nature 190, 502.
    2. Kagawa, Y. and Racker, E. (1966) J. Biol. Chem241, 2461.
    3. Kim, S.B. and Berdanier, C.D. (1999) Am. J. Physiol. Endocrinol. Metab277, E702.
    4. Cunningham, C.C. and George, D.T. (1975) J. Biol. Chem250, 2036.
    5. Arato-Oshima, T. et al. (1996) J. Biol. Chem271, 25604.
    6. Fortes, P.A. and Lee, J.A. (1984) J. Biol. Chem259, 11176.
    7. Garrahan, P.J. and Glynn, I.M. (1967) J. Physiol192, 217.
    8. Cornelius, F. and Skou, J.C. (1985) Biochim. Biophys. Acta. 818, 211.
    9. Martin, W.H. et al. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 9881.
    10. Hegyvary, C. (1976) Biochim. Biophys. Acta. 422, 365.
    11. Fahn, S. et al. (1966) J. Biol. Chem241, 1882.
    12. Fahn, S. et al. (1968) J. Biol. Chem243, 1993.
    13. Inturrisi, C.E. and Titus, E. (1968) Mol. Pharmacol4, 591.
    Scientific background

    Oligomycin is a natural antibiotic isolated from Streptomyces diastatochromogenes which inhibits mitochondrial H+-ATP synthase. It is primarily found to act as an inhibitor of mitochondrial respiration and swelling. This antibiotic is widely used as an inhibitor of oxidative phosphorylation.1-2

    Oligomycin inhibits the H+- ATP-synthase by binding to the Oligomycin sensitivity-conferring protein (OSCP) at the F(o) subunits 6 and 9 which are found in the stalk of the F1F0-ATPase complex. This binding blocks the proton conductance and inhibits the synthesis of mitochondrial ATP.3-4 Because of its activity, it can also be used to reduce the number of parameters (such as ER Ca2+ release, exocytotoxicity and apopotosis) which are affected by mitochondrial depolarization.5

    Oligomycin, at high concentrations may also inhibit the plasma membrane Na+-K+-ATPase. Interaction of Oligomycin with the Na+ occlusion site on the extracellular side of Na/K-ATPase, delays Na+ release to the extracellular side without inducing a conformational change. Although Oligomycin stimulated Na+ binding to Na+/K+-ATPase, it inhibited Na+/Na+ exchange, and did not affect either Na+-dependent AD/-ATP exchange or K+-dependent phosphatase activity.6-16

    These inhibitory properties have been attributed to its ability to occlude the transition of E1~P (high energy intermediate) to E2-P (low energy intermediate) by oligomycin binding to E1~P.17

    Last update: 24/01/2020

    Oligomycin (#O-500) is a highly pure, natural, and biologically active compound.

    For research purposes only, not for human use



    Scientific Background


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