This product is freeze dried. All water molecules have been removed.
Every lot is tried & tested in a relevant biological assay.
- Alomone Labs Oligomycin inhibits ATP-dependent adipogenesis in 3T3-L1 cells.Cells were induced to undergo adipocyte differentiation (A-D) for 5 days. Different concentrations of Oligomycin (#O-500) were then added (B, 1 µg/ml; C, 2 µg/ml and D, 10 µg/ml). After 10 days, the cells were stained with Oil Red O and visualized under light microscopy.
- Chappell, J.B. and Greville, G.D. (1961) Nature 190, 502.
- Kagawa, Y. and Racker, E. (1966) J. Biol. Chem. 241, 2461.
- Kim, S.B. and Berdanier, C.D. (1999) Am. J. Physiol. Endocrinol. Metab. 277, E702.
- Cunningham, C.C. and George, D.T. (1975) J. Biol. Chem. 250, 2036.
- Arato-Oshima, T. et al. (1996) J. Biol. Chem. 271, 25604.
- Fortes, P.A. and Lee, J.A. (1984) J. Biol. Chem. 259, 11176.
- Garrahan, P.J. and Glynn, I.M. (1967) J. Physiol. 192, 217.
- Cornelius, F. and Skou, J.C. (1985) Biochim. Biophys. Acta. 818, 211.
- Martin, W.H. et al. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 9881.
- Hegyvary, C. (1976) Biochim. Biophys. Acta. 422, 365.
- Fahn, S. et al. (1966) J. Biol. Chem. 241, 1882.
- Fahn, S. et al. (1968) J. Biol. Chem. 243, 1993.
- Inturrisi, C.E. and Titus, E. (1968) Mol. Pharmacol. 4, 591.
Oligomycin is a natural antibiotic isolated from Streptomyces diastatochromogenes which inhibits mitochondrial H+-ATP synthase. It is primarily found to act as an inhibitor of mitochondrial respiration and swelling. This antibiotic is widely used as an inhibitor of oxidative phosphorylation.1-2
Oligomycin inhibits the H+- ATP-synthase by binding to the Oligomycin sensitivity-conferring protein (OSCP) at the F(o) subunits 6 and 9 which are found in the stalk of the F1F0-ATPase complex. This binding blocks the proton conductance and inhibits the synthesis of mitochondrial ATP.3-4 Because of its activity, it can also be used to reduce the number of parameters (such as ER Ca2+ release, exocytotoxicity and apopotosis) which are affected by mitochondrial depolarization.5
Oligomycin, at high concentrations may also inhibit the plasma membrane Na+-K+-ATPase. Interaction of Oligomycin with the Na+ occlusion site on the extracellular side of Na/K-ATPase, delays Na+ release to the extracellular side without inducing a conformational change. Although Oligomycin stimulated Na+ binding to Na+/K+-ATPase, it inhibited Na+/Na+ exchange, and did not affect either Na+-dependent AD/-ATP exchange or K+-dependent phosphatase activity.6-16
These inhibitory properties have been attributed to its ability to occlude the transition of E1~P (high energy intermediate) to E2-P (low energy intermediate) by oligomycin binding to E1~P.17
Oligomycin (#O-500) is a highly pure, natural, and biologically active compound.