- Method of UseDissolve the lyophilized powder by adding 20µl double distilled water (DDW). Heat the solubilized product to 100°C for 5 minutes. Load 10µl of the solution into an SDS-PAGE lane alongside the experimental samples. Run the gel and perform Western blot with an Anti-proBDNF antibody as per your usual protocol. For example, our own Anti-proBDNF Antibody (ANT-006).
- Expected ResultSingle band detected at 26kD.
- Western blot analysis using proBDNF Positive ControlLanes 1 and 2: 10µl (corresponding to 50ng) per lane of proBDNF Positive Control for Western Blot (#PCP-B257), was run in a SDS-PAGE and detected in a Western blot using Anti-proBDNF Antibody (#ANT-006), (1:200).
proBDNF Positive Control for Western Blot (#PCP-B257), is a recombinant, highly purified proBDNF protein in a ready-to-use single reagent format. This makes it a good positive control by loading it alongside the experimental lysates or samples in a SDS-PAGE. This product enables to check whether the primary and secondary antibodies are working as expected and also visualize a reference of the expected molecular weight of the target protein.