Overview
Cat #: BLP-CC029
Type: Synthetic peptide
Form: Lyophilized powder
Rat TRPV1/VR1 (extracellular) Blocking Peptide (#BLP-CC029) is the original antigen used for immunization during Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029) generation. The blocking peptide binds and ‘blocks’ Anti-Rat TRPV1/VR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of TRPV1 in rat DRG:1. Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029), (1:200).
2. Anti-Rat TRPV1 (VR1) (extracellular) Antibody, preincubated with Rat TRPV1/VR1 (extracellular) Blocking Peptide (#BLP-CC029). - Expression of TRPV1 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029), (1:100) followed by goat anti-rabbit-ATTO Fluor-594 secondary antibody (red). TRPV1 is expressed in medium and small DRG neurons. Hoechst 33342 is used as the counterstain (blue).
Properties
Sequence
Accession (Uniprot) Number O35433
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Related Products
Related Products
Shipping and Ordering information
Need Help With This Product?
Our reagent specialists are here to help you find the best product for your application. Please call or email us and we will be happy to help you find the right product for the job.
Call us at
+972 2587 2202 or Email Us