Rat TRPV1/VR1 (extracellular) Blocking Peptide (#BLP-CC029) is the original antigen used for immunization during Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029) generation. The blocking peptide binds and ‘blocks’ Anti-Rat TRPV1/VR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of TRPV1 in rat DRG:
- Expression of TRPV1 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029), (1:100) followed by goat anti-rabbit-ATTO Fluor-594 secondary antibody (red). TRPV1 is expressed in medium and small DRG neurons. Hoechst 33342 is used as the counterstain (blue).
- (C)NSLPMESTPHK*SRGS, corresponding to amino acid residues 605-619 of rat TRPV1 with replacement of cysteine 616 (C616) with serine (*S) (Accession O35433).